CaMello-XR Enables Visualization and Optogenetic Control of G Signals and Receptor Trafficking in GPCR-specific Domains

Overview

Journal Commun Biol

Specialty Biology

Date 2019 Feb 23

PMID 30793039

Citations 13

Authors

Dennis Eickelbeck

Raziye Karapinar

Alexander Jack

Sandra T Suess

Ruxandra Barzan

Zohre Azimi

Tatjana Surdin

Michelle Grommke

Melanie D Mark

Klaus Gerwert

Dirk Jancke

Petra Wahle

Katharina Spoida

Stefan Herlitze

Affiliations

Soon will be listed here.

Abstract

The signal specificity of G protein-coupled receptors (GPCRs) including serotonin receptors (5-HT-R) depends on the trafficking and localization of the GPCR within its subcellular signaling domain. Visualizing traffic-dependent GPCR signals in neurons is difficult, but important to understand the contribution of GPCRs to synaptic plasticity. We engineered CaMello (Ca-melanopsin-local-sensor) and CaMello-5HT for visualization of traffic-dependent Ca signals in 5-HT-R domains. These constructs consist of the light-activated G coupled melanopsin, mCherry and GCaMP6m for visualization of Ca signals and receptor trafficking, and the 5-HT C-terminus for targeting into 5-HT-R domains. We show that the specific localization of the GPCR to its receptor domain drastically alters the dynamics and localization of the intracellular Ca signals in different neuronal populations in vitro and in vivo. The CaMello method may be extended to every GPCR coupling to the G pathway to help unravel new receptor-specific functions in respect to synaptic plasticity and GPCR localization.

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