Surface Tethering of Stem Cells with HO-responsive Anti-oxidizing Colloidal Particles for Protection Against Oxidation-induced Death

Overview

Journal Biomaterials

Specialty Biomedical Engineering

Date 2019 Feb 21

PMID 30784768

Citations 11

Authors

Jye Yng Teo

Yongbeom Seo

Eunkyung Ko

Jiayu Leong

Yu-Tong Hong

Yi Yan Yang

Hyunjoon Kong

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells are the new generation of medicine for treating numerous vascular diseases and tissue defects because of their ability to secrete therapeutic factors. Poor cellular survival in an oxidative diseased tissue, however, hinders the therapeutic efficacy. To this end, we hypothesized that tethering the surface of stem cells with colloidal particles capable of discharging antioxidant cargos in response to elevated levels of hydrogen peroxide (HO) would maintain survival and therapeutic activity of the stem cells. We examined this hypothesis by encapsulating epigallocatechin gallate (EGCG) and manganese oxide (MnO) nanocatalysts into particles comprising poly(d,l-lactide-co-glycolide)-block-hyaluronic acid. The MnO nanocatalysts catalyzed the decomposition of HO into oxygen gas, which increased the internal pressure of particles and accelerated the release of EGCG by 1.5-fold. Consequently, stem cells exhibited 1.2-fold higher metabolic activity and 2.8-fold higher secretion level of pro-angiogenic factor in sub-lethal HO concentrations. These stem cells, in turn, performed a greater angiogenic potential with doubled number of newly formed mature blood vessels. We envisage that the results of this study will contribute to improving the therapeutic efficacy of a wide array of stem cells.

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References

Roy S, Khanna S, Nallu K, Hunt T, Sen C . Dermal wound healing is subject to redox control. Mol Ther. 2005; 13(1):211-20. PMC: 1389791. DOI: 10.1016/j.ymthe.2005.07.684. View

Nauta A, Fibbe W . Immunomodulatory properties of mesenchymal stromal cells. Blood. 2007; 110(10):3499-506. DOI: 10.1182/blood-2007-02-069716. View

Makadia H, Siegel S . Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier. Polymers (Basel). 2012; 3(3):1377-1397. PMC: 3347861. DOI: 10.3390/polym3031377. View

Solaini G, Baracca A, Lenaz G, Sgarbi G . Hypoxia and mitochondrial oxidative metabolism. Biochim Biophys Acta. 2010; 1797(6-7):1171-7. DOI: 10.1016/j.bbabio.2010.02.011. View

Saldana L, Valles G, Bensiamar F, Mancebo F, Garcia-Rey E, Vilaboa N . Paracrine interactions between mesenchymal stem cells and macrophages are regulated by 1,25-dihydroxyvitamin D3. Sci Rep. 2017; 7(1):14618. PMC: 5668416. DOI: 10.1038/s41598-017-15217-8. View

Deponte M . Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes. Biochim Biophys Acta. 2012; 1830(5):3217-66. DOI: 10.1016/j.bbagen.2012.09.018. View

Mildmay-White A, Khan W . Cell Surface Markers on Adipose-Derived Stem Cells: A Systematic Review. Curr Stem Cell Res Ther. 2016; 12(6):484-492. DOI: 10.2174/1574888X11666160429122133. View

Guzy R, Schumacker P . Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia. Exp Physiol. 2006; 91(5):807-19. DOI: 10.1113/expphysiol.2006.033506. View

Coombes E, Jiang J, Chu X, Inoue K, Seeds J, Branigan D . Pathophysiologically relevant levels of hydrogen peroxide induce glutamate-independent neurodegeneration that involves activation of transient receptor potential melastatin 7 channels. Antioxid Redox Signal. 2010; 14(10):1815-27. PMC: 3078500. DOI: 10.1089/ars.2010.3549. View

10.

Wang X, Zhao T, Huang W, Wang T, Qian J, Xu M . Hsp20-engineered mesenchymal stem cells are resistant to oxidative stress via enhanced activation of Akt and increased secretion of growth factors. Stem Cells. 2009; 27(12):3021-31. PMC: 2806498. DOI: 10.1002/stem.230. View

11.

Birben E, Sahiner U, Sackesen C, Erzurum S, Kalayci O . Oxidative stress and antioxidant defense. World Allergy Organ J. 2012; 5(1):9-19. PMC: 3488923. DOI: 10.1097/WOX.0b013e3182439613. View

12.

Gyongyosi M, Blanco J, Marian T, Tron L, Petnehazy O, Petrasi Z . Serial noninvasive in vivo positron emission tomographic tracking of percutaneously intramyocardially injected autologous porcine mesenchymal stem cells modified for transgene reporter gene expression. Circ Cardiovasc Imaging. 2009; 1(2):94-103. PMC: 3053595. DOI: 10.1161/CIRCIMAGING.108.797449. View

13.

Lu J, Wang X, Marin-Muller C, Wang H, Lin P, Yao Q . Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Rev Mol Diagn. 2009; 9(4):325-41. PMC: 2701163. DOI: 10.1586/erm.09.15. View

14.

Gauron C, Rampon C, Bouzaffour M, Ipendey E, Teillon J, Volovitch M . Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed. Sci Rep. 2013; 3:2084. PMC: 3694286. DOI: 10.1038/srep02084. View

15.

Lesley J, Hascall V, Tammi M, HYMAN R . Hyaluronan binding by cell surface CD44. J Biol Chem. 2000; 275(35):26967-75. DOI: 10.1074/jbc.M002527200. View

16.

Schafer R, Spohn G, Baer P . Mesenchymal Stem/Stromal Cells in Regenerative Medicine: Can Preconditioning Strategies Improve Therapeutic Efficacy?. Transfus Med Hemother. 2016; 43(4):256-267. PMC: 5040900. DOI: 10.1159/000447458. View

17.

Yagi H, Tan J, Tuan R . Polyphenols suppress hydrogen peroxide-induced oxidative stress in human bone-marrow derived mesenchymal stem cells. J Cell Biochem. 2012; 114(5):1163-73. DOI: 10.1002/jcb.24459. View

18.

Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe S . Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-54. PMC: 4044300. DOI: 10.1152/physrev.00040.2012. View

19.

Weisburg J, Weissman D, Sedaghat T, Babich H . In vitro cytotoxicity of epigallocatechin gallate and tea extracts to cancerous and normal cells from the human oral cavity. Basic Clin Pharmacol Toxicol. 2004; 95(4):191-200. DOI: 10.1111/j.1742-7843.2004.pto_950407.x. View

20.

Kyurkchiev D, Bochev I, Ivanova-Todorova E, Mourdjeva M, Oreshkova T, Belemezova K . Secretion of immunoregulatory cytokines by mesenchymal stem cells. World J Stem Cells. 2014; 6(5):552-70. PMC: 4178255. DOI: 10.4252/wjsc.v6.i5.552. View