Curcumae Radix Extract Decreases Mammary Tumor-Derived Lung Metastasis Via Suppression of C-C Chemokine Receptor Type 7 Expression

Overview

Journal Nutrients

Date 2019 Feb 21

PMID 30781353

Citations 13

Authors

Pelin Kaya

Sang R Lee

Young Ho Lee

Sun Woo Kwon

Hyun Yang

Hye Won Lee

Eui-Ju Hong

Affiliations

Soon will be listed here.

Abstract

Curcumae radix is the dry root of (turmeric) that can be used either as a spice or traditional medicine. The aim of this study was to investigate the survival benefits and the anti-metastatic activity of curcumae radix extract (CRE) in MCF7 cells and in MMTV-PyMT transgenic mice-a mouse model of breast cancer metastasis. In vitro wound scratch assay revealed that CRE treatment inhibited cell motility and cell migration in a dose-dependent manner. To investigate the effect of CRE in breast cancer metastasis, MMTV-PyMT transgenic female virgin mice were used and randomly divided into two groups. For survival curve analysis, CRE was administered in a dose of 50 mg/kg to 8⁻20-week-old mice. Interestingly, CRE treatment significantly increased the median and prolonged survival of MMTV-PyMT mice. Furthermore, CRE treatment decreased tumor burden and inhibited cell proliferation in primary breast tumor, and also suppressed mammary tumor-derived lung metastasis. The size of the lung metastases substantially decreased in the CRE-treated group compared with the ones in the control group. Curcumae radix extract showed anti-metastatic activity through regulating the expression of metastasis markers including C-C Chemokine Receptor Type 7, Matrix Metalloproteinase 9 and the proto-oncogenes c-fos and c-jun. We demonstrated that these metastatic regulators were decreased when CCR7 expression was suppressed in MCF7 cells transfected with CCR7 siRNA. The results of this study show that curcumae radix exerts antitumor and anti-metastatic activities, and we suggest that curcumae radix might be a potential supplement for the treatment and prevention of breast cancer metastasis.

Citing Articles

The role and mechanism of "eight famous herbals in Zhejiang" in cancer via network pharmacology and experimental validation.

Ni Z, Zhang H, Chen F, Yang M, Yang L, Zhou Y Front Oncol. 2024; 14:1475000.

PMID: 39628999 PMC: 11612504. DOI: 10.3389/fonc.2024.1475000.

A Strategy based on Bioinformatics and Machine Learning Algorithms Reveals Potential Mechanisms of Shelian Capsule against Hepatocellular Carcinoma.

Zhou X, Tan F, Zhang S, Wang A, Zhang T Curr Pharm Des. 2024; 30(5):377-405.

PMID: 38310567 DOI: 10.2174/0113816128284465240108071554.

A real-world study and network pharmacology analysis of EGFR-TKIs combined with ZLJT to delay drug resistance in advanced lung adenocarcinoma.

Han X, Liang L, He C, Ren Q, Su J, Cao L BMC Complement Med Ther. 2023; 23(1):422.

PMID: 37990309 PMC: 10664478. DOI: 10.1186/s12906-023-04213-3.

Curcumin and chemokines: mechanism of action and therapeutic potential in inflammatory diseases.

Sadeghi M, Dehnavi S, Asadirad A, Xu S, Majeed M, Jamialahmadi T Inflammopharmacology. 2023; 31(3):1069-1093.

PMID: 36997729 PMC: 10062691. DOI: 10.1007/s10787-023-01136-w.

Comparative Analysis of Volatile Constituents in Root Tuber and Rhizome of L. Using Fingerprints and Chemometrics Approaches on Gas Chromatography-Mass Spectrometry.

Tang G, Shi Y, Gao W, Li M, Li P, Yang H Molecules. 2022; 27(10).

PMID: 35630672 PMC: 9145967. DOI: 10.3390/molecules27103196.

References

Cardiff R, Anver M, Gusterson B, Hennighausen L, Jensen R, Merino M . The mammary pathology of genetically engineered mice: the consensus report and recommendations from the Annapolis meeting. Oncogene. 2000; 19(8):968-88. DOI: 10.1038/sj.onc.1203277. View

Shao Z, Shen Z, Liu C, Sartippour M, Go V, Heber D . Curcumin exerts multiple suppressive effects on human breast carcinoma cells. Int J Cancer. 2002; 98(2):234-40. DOI: 10.1002/ijc.10183. View

Ramachandran C, Fonseca H, Jhabvala P, Escalon E, Melnick S . Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line. Cancer Lett. 2002; 184(1):1-6. DOI: 10.1016/s0304-3835(02)00192-1. View

Hayashi S, Eguchi H, Tanimoto K, Yoshida T, Omoto Y, Inoue A . The expression and function of estrogen receptor alpha and beta in human breast cancer and its clinical application. Endocr Relat Cancer. 2003; 10(2):193-202. DOI: 10.1677/erc.0.0100193. View

Guy C, Cardiff R, Muller W . Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease. Mol Cell Biol. 1992; 12(3):954-61. PMC: 369527. DOI: 10.1128/mcb.12.3.954-961.1992. View

Williams T, Medina F, Badano I, Hazan R, Hutchinson J, Muller W . Caveolin-1 gene disruption promotes mammary tumorigenesis and dramatically enhances lung metastasis in vivo. Role of Cav-1 in cell invasiveness and matrix metalloproteinase (MMP-2/9) secretion. J Biol Chem. 2004; 279(49):51630-46. DOI: 10.1074/jbc.M409214200. View

Riol-Blanco L, Sanchez-Sanchez N, Torres A, Tejedor A, Narumiya S, Corbi A . The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed. J Immunol. 2005; 174(7):4070-80. DOI: 10.4049/jimmunol.174.7.4070. View

Lantz R, Chen G, Solyom A, Jolad S, Timmermann B . The effect of turmeric extracts on inflammatory mediator production. Phytomedicine. 2005; 12(6-7):445-52. DOI: 10.1016/j.phymed.2003.12.011. View

Minn A, Gupta G, Siegel P, Bos P, Shu W, Giri D . Genes that mediate breast cancer metastasis to lung. Nature. 2005; 436(7050):518-24. PMC: 1283098. DOI: 10.1038/nature03799. View

10.

Ramachandran C, Rodriguez S, Ramachandran R, Nair P, Fonseca H, Khatib Z . Expression profiles of apoptotic genes induced by curcumin in human breast cancer and mammary epithelial cell lines. Anticancer Res. 2005; 25(5):3293-302. View

11.

Cabioglu N, Yazici M, Arun B, Broglio K, Hortobagyi G, Price J . CCR7 and CXCR4 as novel biomarkers predicting axillary lymph node metastasis in T1 breast cancer. Clin Cancer Res. 2005; 11(16):5686-93. DOI: 10.1158/1078-0432.CCR-05-0014. View

12.

Aggarwal B, Shishodia S, Takada Y, Banerjee S, Newman R, Bueso-Ramos C . Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice. Clin Cancer Res. 2005; 11(20):7490-8. DOI: 10.1158/1078-0432.CCR-05-1192. View

13.

Yiu G, Toker A . NFAT induces breast cancer cell invasion by promoting the induction of cyclooxygenase-2. J Biol Chem. 2006; 281(18):12210-7. DOI: 10.1074/jbc.M600184200. View

14.

Redondo-Munoz J, Terol M, Garcia-Marco J, Garcia-Pardo A . Matrix metalloproteinase-9 is up-regulated by CCL21/CCR7 interaction via extracellular signal-regulated kinase-1/2 signaling and is involved in CCL21-driven B-cell chronic lymphocytic leukemia cell invasion and migration. Blood. 2007; 111(1):383-6. DOI: 10.1182/blood-2007-08-107300. View

15.

Chuang H, Lee E, Liu Y, Lee D, Ideker T . Network-based classification of breast cancer metastasis. Mol Syst Biol. 2007; 3:140. PMC: 2063581. DOI: 10.1038/msb4100180. View

16.

Lu B, Xu L, Yu L, Zhang L . Extract of radix curcumae prevents gastric cancer in rats. Digestion. 2008; 77(2):87-91. DOI: 10.1159/000122228. View

17.

Xiao Y, Yang F, Li S, Hu G, Lee S, Wang Y . Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells. World J Gastroenterol. 2008; 14(27):4309-18. PMC: 2731181. DOI: 10.3748/wjg.14.4309. View

18.

Wang S, Yuan Y, Liao L, Kuang S, Tien J, OMalley B . Disruption of the SRC-1 gene in mice suppresses breast cancer metastasis without affecting primary tumor formation. Proc Natl Acad Sci U S A. 2008; 106(1):151-6. PMC: 2629242. DOI: 10.1073/pnas.0808703105. View

19.

Yodkeeree S, Ampasavate C, Sung B, Aggarwal B, Limtrakul P . Demethoxycurcumin suppresses migration and invasion of MDA-MB-231 human breast cancer cell line. Eur J Pharmacol. 2009; 627(1-3):8-15. DOI: 10.1016/j.ejphar.2009.09.052. View

20.

Lim C, Ky N, Ng H, Hamza M, Zhao Y . Curcuma wenyujin extract induces apoptosis and inhibits proliferation of human cervical cancer cells in vitro and in vivo. Integr Cancer Ther. 2010; 9(1):36-49. DOI: 10.1177/1534735409359773. View