Mitochondrial Small Heat Shock Protein Mediates Seed Germination Via Thermal Sensing

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2019 Feb 16

PMID 30765516

Citations 38

Authors

Wei Ma

Xueying Guan

Jie Li

Ronghui Pan

Luyao Wang

Fengjun Liu

Hongyu Ma

Shuijin Zhu

Jin Hu

Yong-Ling Ruan

Xiaoya Chen

Tianzhen Zhang

Affiliations

Soon will be listed here.

Abstract

Seed germination is an energy demanding process that requires functional mitochondria upon imbibition. However, how mitochondria fine tune seed germination, especially in response to the dynamics of environmental temperature, remains largely unknown at the molecular level. Here, we report a mitochondrial matrix-localized heat shock protein GhHSP24.7, that regulates seed germination in a temperature-dependent manner. Suppression of renders the seed insensitive to temperature changes and delays germination. We show that GhHSP24.7 competes with GhCCMH to bind to the maturation subunit protein GhCcmF to form cytochrome C/C (CytC/C) in the mitochondrial electron transport chain. GhHSP24.7 modulates CytC/C production to induce reactive oxygen species (ROS) generation, which consequently accelerates endosperm rupture and promotes seed germination. Overexpression of 's homologous genes can accelerate seed germination in and tomato, indicating its conserved function across plant species. Therefore, is a critical factor that positively controls seed germination via temperature-dependent ROS generation.

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