Comparative Analysis of Mutational Robustness of the Intrinsically Disordered Viral Protein VPg and of Its Interactor EIF4E

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Feb 15

PMID 30763345

Citations 7

Authors

Jocelyne Walter

Justine Charon

Yihua Hu

Joy Lachat

Thomas Leger

Guillaume Lafforgue

Amandine Barra

Thierry Michon

Affiliations

Soon will be listed here.

Abstract

Conformational intrinsic disorder is a feature present in many virus proteins. Intrinsically disordered regions (IDRs) have weaker structural requirement than ordered regions and mutations in IDRs could have a lower impact on the virus fitness. This could favor its exploration of adaptive solutions. The potyviral protein VPg contains IDRs with determinants for adaptation to its host plant. To experimentally assess whether IDRs are more resistant to mutations than ordered regions, the biologically relevant interaction between mutant libraries of both VPg and the eukaryotic translation initiation factor 4E (eIF4E) and their respective wild type partner was examined using yeast two hybrid assay. Our data shows that VPg is significantly more robust to mutations than eIF4E and as such belongs to a particular class of intrinsically disordered proteins. This result is discussed from the standpoint of IDRs involvement in the virus adaptive processes.

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