Hydroxyectoine Protects Mn-depleted Photosystem II Against Photoinhibition Acting As a Source of Electrons

Overview

Journal Photosynth Res

Publisher Springer

Date 2019 Feb 1

PMID 30701483

Citations 2

Authors

D V Yanykin

M Malferrari

S Rapino

G Venturoli

A Yu Semenov

M D Mamedov

Affiliations

Soon will be listed here.

Abstract

In the present study, we have investigated the effect of hydroxyectoine (Ect-OH), a heterocyclic amino acid, on oxygen evolution in photosystem II (PS II) membrane fragments and on photoinhibition of Mn-depleted PS II (apo-WOC-PS II) preparations. The degree of photoinhibition of apo-WOC-PS II preparations was estimated by the loss of the capability of exogenous electron donor (sodium ascorbate) to restore the amplitude of light-induced changes of chlorophyll fluorescence yield (∆F). It was found that Ect-OH (i) stimulates the oxygen-evolving activity of PS II, (ii) accelerates the electron transfer from exogenous electron donors (K[Fe(CN)], DPC, TMPD, Fe, and Mn) to the reaction center of apo-WOC-PS II, (iii) enhances the protective effect of exogenous electron donors against donor-side photoinhibition of apo-WOC-PS II preparations. It is assumed that Ect-OH can serve as an artificial electron donor for apo-WOC-PS II, which does not directly interact with either the donor or acceptor side of the reaction center. We suggest that the protein conformation in the presence of Ect-OH, which affects the extent of hydration, becomes favorable for accepting electrons from exogenous donors. To our knowledge, this is the first study dealing with redox activity of Ect-OH towards photosynthetic pigment-protein complexes.

Citing Articles

Effects of Novel Photosynthetic Inhibitor [CuL]Br Complex on Photosystem II Activity in Spinach.

Zharmukhamedov S, Shabanova M, Rodionova M, Huseynova I, Karacan M, Karacan N Cells. 2022; 11(17).

PMID: 36078088 PMC: 9455146. DOI: 10.3390/cells11172680.

The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine.

Richter A, Kobus S, Czech L, Hoeppner A, Zarzycki J, Erb T J Biol Chem. 2020; 295(9):2822-2838.

PMID: 31969391 PMC: 7049965. DOI: 10.1074/jbc.RA119.011277.

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