Open-Source Multiparametric Optocardiography

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jan 26

PMID 30679527

Citations 11

Authors

Brianna Cathey

Sofian Obaid

Alexander M Zolotarev

Roman A Pryamonosov

Roman A Syunyaev

Sharon A George

Igor R Efimov

Affiliations

Soon will be listed here.

Abstract

Since the 1970s fluorescence imaging has become a leading tool in the discovery of mechanisms of cardiac function and arrhythmias. Gradual improvements in fluorescent probes and multi-camera technology have increased the power of optical mapping and made a major impact on the field of cardiac electrophysiology. Tandem-lens optical mapping systems facilitated simultaneous recording of multiple parameters characterizing cardiac function. However, high cost and technological complexity restricted its proliferation to the wider biological community. We present here, an open-source solution for multiple-camera tandem-lens optical systems for multiparametric mapping of transmembrane potential, intracellular calcium dynamics and other parameters in intact mouse hearts and in rat heart slices. This 3D-printable hardware and Matlab-based RHYTHM 1.2 analysis software are distributed under an MIT open-source license. Rapid prototyping permits the development of inexpensive, customized systems with broad functionality, allowing wider application of this technology outside biomedical engineering laboratories.

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