Membrane Reshaping by Micrometric Curvature Sensitive Septin Filaments

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Jan 26

PMID 30679428

Citations 40

Authors

Alexandre Beber

Cyntia Taveneau

Manuela Nania

Feng-Ching Tsai

Aurelie Di Cicco

Patricia Bassereau

Daniel Levy

Joao T Cabral

Herve Isambert

Stephanie Mangenot

Aurelie Bertin

Affiliations

Soon will be listed here.

Abstract

Septins are cytoskeletal filaments that assemble at the inner face of the plasma membrane. They are localized at constriction sites and impact membrane remodeling. We report in vitro tools to examine how yeast septins behave on curved and deformable membranes. Septins reshape the membranes of Giant Unilamellar Vesicles with the formation of periodic spikes, while flattening smaller vesicles. We show that membrane deformations are associated to preferential arrangement of septin filaments on specific curvatures. When binding to bilayers supported on custom-designed periodic wavy patterns displaying positive and negative micrometric radii of curvatures, septin filaments remain straight and perpendicular to the curvature of the convex parts, while bending negatively to follow concave geometries. Based on these results, we propose a theoretical model that describes the deformations and micrometric curvature sensitivity observed in vitro. The model captures the reorganizations of septin filaments throughout cytokinesis in vivo, providing mechanistic insights into cell division.

Citing Articles

Lipid packing and local geometry influence septin curvature sensing.

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Biomimetic Materials to Fabricate Artificial Cells.

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