Degradation of Endogenous Proteins and Generation of a Null-like Phenotype in Zebrafish Using Trim-Away Technology

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2019 Jan 25

PMID 30674345

Citations 15

Authors

Xiao Chen

Mi Liu

Hongyan Lou

Yiyi Lu

Meng-Tao Zhou

Rongying Ou

Yunsheng Xu

Kai-Fu Tang

Affiliations

Soon will be listed here.

Abstract

Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.

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