Challenges in Quantifying Cytosine Methylation in the HIV Provirus

Overview

Journal mBio

Publisher American Society for Microbiology

Specialty Microbiology

Date 2019 Jan 24

PMID 30670613

Citations 8

Authors

Sarah A LaMere

Antoine Chaillon

Christina Huynh

Davey M Smith

Sara Gianella

Affiliations

Soon will be listed here.

Abstract

DNA methylation is an epigenetic mechanism most commonly associated with transcriptional repression. While it is clear that DNA methylation can silence HIV proviral expression in latency models, its correlation with HIV persistence and expression is ambiguous, particularly in persons living with HIV (PLWH) receiving antiretroviral therapy (ART). Several factors potentially contribute to discrepancies between results in the literature, including differences in integration sites, functional proviral load, sampling bias, and stochastic PCR amplification. Recent studies into genomic features of cytosine methylation sites in mammalian genes offer potentially significant insights into this mechanism. Here, we discuss the importance of these factors in the context of the HIV.

Citing Articles

Deciphering DNA Methylation in HIV Infection.

Arumugam T, Ramphal U, Adimulam T, Chinniah R, Ramsuran V Front Immunol. 2021; 12:795121.

PMID: 34925380 PMC: 8674454. DOI: 10.3389/fimmu.2021.795121.

Clonal Expansion of Infected CD4+ T Cells in People Living with HIV.

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Targeting Epigenetics to Cure HIV-1: Lessons From (and for) Cancer Treatment.

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CpG Methylation Profiles of HIV-1 Pro-Viral DNA in Individuals on ART.

Boltz V, Ceriani C, Rausch J, Shao W, Bale M, Keele B Viruses. 2021; 13(5).

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Balance between Retroviral Latency and Transcription: Based on HIV Model.

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