The Timing of Cumulus Cell Removal for Intracytoplasmic Sperm Injection Influences the Capability of Embryonic Development

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2019 Jan 19

PMID 30655729

Citations 11

Authors

Satoshi Mizuno

Yuko Ishikawa

Hiroshi Matsumoto

Manabu Sato

Mamoru Ida

Aisaku Fukuda

Yoshiharu Morimoto

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine whether the presence of intact cumulus cells during the preincubation period for ICSI should be considered as a critical factor in fertilization and embryonic development.

Methods: The cohort of this prospective randomized study was limited to infertile women younger than 39 years of age who underwent controlled ovarian stimulation for ICSI between October 2013 and May 2015 and whose embryos were to be incubated until day 5. Women with estradiol levels of <2000 pmol/L on the day of HCG injection were excluded. Cumulus cells were removed immediately after OPU in Group A and at 120 minutes after OPU in Group B. ICSI was performed with all mature oocytes, and fertilized oocytes were cultured to the blastocyst stage. Maturation, fertilization, blastocyst, good quality blastocyst, pregnancy, live birth, and miscarriage rates were compared.

Results: There were no significant differences in maturation, fertilization, blastocyst, pregnancy, live birth, or miscarriage rates between Groups A and B. However, the percentage of good quality blastocysts was significantly higher in Group B than Group A (52.0% vs 33.1%).

Conclusions: Intact cumulus cells should be maintained during the preincubation period, as they are important to embryonic development after fertilization.

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References

Ho J, Chen M, Yi Y, Guu H, Ho E . The effect of preincubation period of oocytes on nuclear maturity, fertilization rate, embryo quality, and pregnancy outcome in IVF and ICSI. J Assist Reprod Genet. 2003; 20(9):358-64. PMC: 3455840. DOI: 10.1023/a:1025476910771. View

Kikuchi K, Naito K, Noguchi J, Shimada A, Kaneko H, Yamashita M . Maturation/M-phase promoting factor: a regulator of aging in porcine oocytes. Biol Reprod. 2000; 63(3):715-22. DOI: 10.1095/biolreprod63.3.715. View

Mizutani E, Jiang J, Mizuno S, Tomioka I, Shinozawa T, Kobayashi J . Determination of optimal conditions for parthenogenetic activation and subsequent development of rat oocytes in vitro. J Reprod Dev. 2004; 50(1):139-46. DOI: 10.1262/jrd.50.139. View

Qiao T, Liu N, Miao D, Zhang X, Han D, Ge L . Cumulus cells accelerate aging of mouse oocytes by secreting a soluble factor(s). Mol Reprod Dev. 2007; 75(3):521-8. DOI: 10.1002/mrd.20779. View

Ka H, Sawai K, Wang W, Im K, Niwa K . Amino acids in maturation medium and presence of cumulus cells at fertilization promote male pronuclear formation in porcine oocytes matured and penetrated in vitro. Biol Reprod. 1998; 57(6):1478-83. DOI: 10.1095/biolreprod57.6.1478. View

Miao Y, Liu X, Qiao T, Miao D, Luo M, Tan J . Cumulus cells accelerate aging of mouse oocytes. Biol Reprod. 2005; 73(5):1025-31. DOI: 10.1095/biolreprod.105.043703. View

Hassan H . Cumulus cell contribution to cytoplasmic maturation and oocyte developmental competence in vitro. J Assist Reprod Genet. 2001; 18(10):539-43. PMC: 3455316. DOI: 10.1023/a:1011901923261. View

Bianchi S, Macchiarelli G, Micara G, Linari A, Boninsegna C, Aragona C . Ultrastructural markers of quality are impaired in human metaphase II aged oocytes: a comparison between reproductive and in vitro aging. J Assist Reprod Genet. 2015; 32(9):1343-58. PMC: 4595403. DOI: 10.1007/s10815-015-0552-9. View

Mizuno S, Ishikawa Y, Matsumoto H, Sato M, Ida M, Fukuda A . The timing of cumulus cell removal for intracytoplasmic sperm injection influences the capability of embryonic development. Reprod Med Biol. 2019; 18(1):111-117. PMC: 6332823. DOI: 10.1002/rmb2.12257. View

10.

Guerin P, El Mouatassim S, Menezo Y . Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update. 2001; 7(2):175-89. DOI: 10.1093/humupd/7.2.175. View

11.

Fisch B, Kaplan-Kraicer R, Amit S, Ovadia J, Tadir Y . The effect of preinsemination interval upon fertilization of human oocytes in vitro. Hum Reprod. 1989; 4(8):954-6. DOI: 10.1093/oxfordjournals.humrep.a137019. View

12.

Jeseta M, Petr J, Krejcova T, Chmelikova E, Jilek F . In vitro ageing of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A. Zygote. 2008; 16(2):145-52. DOI: 10.1017/S0967199408004668. View

13.

Lacham-Kaplan O, Trounson A . Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI. Reprod Biol Endocrinol. 2008; 6:58. PMC: 2636812. DOI: 10.1186/1477-7827-6-58. View

14.

Boerjan M, de Boer P . First cell cycle of zygotes of the mouse derived from oocytes aged postovulation in vivo and fertilized in vivo. Mol Reprod Dev. 1990; 25(2):155-63. DOI: 10.1002/mrd.1080250208. View

15.

Wakayama S, Van Thuan N, Kishigami S, Ohta H, Mizutani E, Hikichi T . Production of offspring from one-day-old oocytes stored at room temperature. J Reprod Dev. 2005; 50(6):627-37. DOI: 10.1262/jrd.50.627. View

16.

Vanderhyden B, Armstrong D . Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biol Reprod. 1989; 40(4):720-8. DOI: 10.1095/biolreprod40.4.720. View

17.

van Steirteghem A, Nagy Z, Joris H, Liu J, Staessen C, Smitz J . High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod. 1993; 8(7):1061-6. DOI: 10.1093/oxfordjournals.humrep.a138192. View

18.

Kikuchi K, Naito K, Noguchi J, Kaneko H, Tojo H . Maturation/M-phase promoting factor regulates aging of porcine oocytes matured in vitro. Cloning Stem Cells. 2002; 4(3):211-22. DOI: 10.1089/15362300260339494. View

19.

van de Velde H, de Vos A, Joris H, Nagy Z, van Steirteghem A . Effect of timing of oocyte denudation and micro-injection on survival, fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1998; 13(11):3160-4. DOI: 10.1093/humrep/13.11.3160. View

20.

Khan I, Staessen C, Van den Abbeel E, Camus M, Wisanto A, Smitz J . Time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in GnRH agonist/HMG-stimulated cycles. Hum Reprod. 1989; 4(8):921-6. DOI: 10.1093/oxfordjournals.humrep.a137013. View