Lineage-Specific Chiral Biases of Human Embryonic Stem Cells During Differentiation

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2019 Jan 11

PMID 30627170

Citations 7

Authors

Kathryn E Worley

Amanda S Chin

Leo Q Wan

Affiliations

Soon will be listed here.

Abstract

Left-right symmetry breaking is a complex developmental process and an important part of embryonic axis development. As of yet, the biophysical mechanism behind LR asymmetry establishment remains elusive for the overall asymmetry of embryos as well as for the organ-specific asymmetry. Here, we demonstrate that inherent cellular chirality is observable in the cells of early embryonic stages using a 3D Matrigel bilayer system. Differentiation of human embryonic stem cells to three lineages corresponding to heart, intestine, and neural tissues demonstrates phenotype-specific inherent chiral biases, complementing the current knowledge regarding organ development. The existence of inherent cellular chirality early in development and its correlation with organ asymmetry implicate cell chirality as a possible regulator in LR symmetry breaking.

Citing Articles

Biomechanical Modeling of Cell Chirality and Symmetry Breaking of Biological Systems.

Rahman T, Peters F, Wan L Mechanobiol Med. 2024; 2(1).

PMID: 38721590 PMC: 11078094. DOI: 10.1016/j.mbm.2024.100038.

Asymmetrical positioning of cell organelles reflects the cell chirality of mouse myoblast cells.

Hachem Z, Hadrian C, Aldbaisi L, Alkaabi M, Wan L, Fan J APL Bioeng. 2024; 8(1):016119.

PMID: 38495528 PMC: 10942803. DOI: 10.1063/5.0189401.

Helical vasculogenesis driven by cell chirality.

Zhang H, Rahman T, Lu S, Adam A, Wan L Sci Adv. 2024; 10(8):eadj3582.

PMID: 38381835 PMC: 10881055. DOI: 10.1126/sciadv.adj3582.

A Micropatterning Assay for Measuring Cell Chirality.

Zhang H, Ronaldson-Bouchard K, Vunjak-Novakovic G, Wan L J Vis Exp. 2022; (181).

PMID: 35343954 PMC: 9008871. DOI: 10.3791/63105.

Cell Chirality as a Novel Measure for Cytotoxicity.

Zhang H, Wan L Adv Biol (Weinh). 2021; 6(1):e2101088.

PMID: 34796704 PMC: 9008805. DOI: 10.1002/adbi.202101088.

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