Ras (proto)oncogene Induces N-linked Carbohydrate Modification: Temporal Relationship with Induction of Invasive Potential

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1988 Nov 1

PMID 3061796

Citations 12

Authors

J G Bolscher

M M Van der Bijl

J J Neefjes

A Hall

L A Smets

H L Ploegh

Affiliations

Soon will be listed here.

Abstract

The effect of expression of the ras oncogene on protein glycosylation was studied. VSV G-protein and class I histocompatibility antigens were analysed to monitor ras-mediated changes in glycosylation. Transient expression of the c-Ha-ras oncogene, introduced into NIH 3T3 cells by the DEAE-dextran method, altered protein glycosylation within 25 h of transfection. The same result was obtained after dexamethasone-induced expression of p21-ras in stable NIH 3T3 transfectants containing either an activated Ha-ras oncogene or a normal N-ras proto-oncogene under control of the glucocorticoid-inducible MMTV promoter. The alteration of cell surface carbohydrates, induced by the ras (proto)oncogene and the subsequent acquisition of invasive potential, occurred prior to morphological transformation.

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