Upconverting Nanoparticle Micro-lightbulbs Designed for Deep Tissue Optical Stimulation and Imaging

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2019 Jan 8

PMID 30615722

Citations 4

Authors

Maysamreza Chamanzar

David J Garfield

Jillian Iafrati

Emory M Chan

Vikaas Sohal

Bruce E Cohen

P James Schuck

Michel M Maharbiz

Affiliations

Soon will be listed here.

Abstract

Optical methods for imaging and stimulation of biological events based on the use of visible light are limited to the superficial layers of tissue due to the significant absorption and scattering of light. Here, we demonstrate the design and implementation of passive micro-structured lightbulbs (MLBs) containing bright-emitting lanthanide-doped upconverting nanoparticles (UCNPs) for light delivery deep into the tissue. The MLBs are realized as cylindrical pillars made of Parylene C polymer that can be implanted deep into the tissue. The encapsulated UCNPs absorb near-infrared (NIR) light at λ = 980 nm, which undergoes much less absorption than the blue light in the brain tissue, and then locally emit blue light (G→H and D→F transitions) that can be used for optogenetic excitation of neurons in the brain. The H→H transition will result in the emission of higher energy NIR photons at λ = 800 nm that can be used for imaging and tracking MLBs through thick tissue.

Citing Articles

Emerging trends in the development of flexible optrode arrays for electrophysiology.

Almasri R, Ladouceur F, Mawad D, Esrafilzadeh D, Firth J, Lehmann T APL Bioeng. 2023; 7(3):031503.

PMID: 37692375 PMC: 10491464. DOI: 10.1063/5.0153753.

Upconverting Nanoparticles as a New Bio-Imaging Strategy-Investigating Intracellular Trafficking of Endogenous Processes in Neural Tissue.

Zajdel K, Janowska J, Frontczak-Baniewicz M, Sypecka J, Sikora B Int J Mol Sci. 2023; 24(2).

PMID: 36674638 PMC: 9866400. DOI: 10.3390/ijms24021122.

Near-Infrared-Triggered Upconverting Nanoparticles for Biomedicine Applications.

Mahata M, De R, Lee K Biomedicines. 2021; 9(7).

PMID: 34210059 PMC: 8301434. DOI: 10.3390/biomedicines9070756.

In vivo deep-tissue microscopy with UCNP/Janus-dendrimers as imaging probes: resolution at depth and feasibility of ratiometric sensing.

Plunkett S, Khatib M, Sencan I, Porter J, Kumar A, Collins J Nanoscale. 2020; 12(4):2657-2672.

PMID: 31939953 PMC: 7101076. DOI: 10.1039/c9nr07778b.

References

Djurisic M, Zochowski M, Wachowiak M, Falk C, Cohen L, Zecevic D . Optical monitoring of neural activity using voltage-sensitive dyes. Methods Enzymol. 2003; 361:423-51. DOI: 10.1016/s0076-6879(03)61022-0. View

Baker B, Kosmidis E, Vucinic D, Falk C, Cohen L, Djurisic M . Imaging brain activity with voltage- and calcium-sensitive dyes. Cell Mol Neurobiol. 2005; 25(2):245-82. PMC: 11529562. DOI: 10.1007/s10571-005-3059-6. View

Yang L, Miklavcic S . Revised Kubelka-Munk theory. III. A general theory of light propagation in scattering and absorptive media. J Opt Soc Am A Opt Image Sci Vis. 2005; 22(9):1866-73. DOI: 10.1364/josaa.22.001866. View

Soltani M, Yegnanarayanan S, Adibi A . Ultra-high Q planar silicon microdisk resonators for chip-scale silicon photonics. Opt Express. 2009; 15(8):4694-704. DOI: 10.1364/oe.15.004694. View

Chan E, Xu C, Mao A, Han G, Owen J, Cohen B . Reproducible, high-throughput synthesis of colloidal nanocrystals for optimization in multidimensional parameter space. Nano Lett. 2010; 10(5):1874-85. DOI: 10.1021/nl100669s. View

Zorzos A, Boyden E, Fonstad C . Multiwaveguide implantable probe for light delivery to sets of distributed brain targets. Opt Lett. 2010; 35(24):4133-5. PMC: 3010404. DOI: 10.1364/OL.35.004133. View

Deisseroth K . Optogenetics. Nat Methods. 2010; 8(1):26-9. PMC: 6814250. DOI: 10.1038/nmeth.f.324. View

Boyden E . A history of optogenetics: the development of tools for controlling brain circuits with light. F1000 Biol Rep. 2011; 3:11. PMC: 3155186. DOI: 10.3410/B3-11. View

Kobat D, Horton N, Xu C . In vivo two-photon microscopy to 1.6-mm depth in mouse cortex. J Biomed Opt. 2011; 16(10):106014. DOI: 10.1117/1.3646209. View

10.

Ledochowitsch P, Olivero E, Blanche T, Maharbiz M . A transparent μECoG array for simultaneous recording and optogenetic stimulation. Annu Int Conf IEEE Eng Med Biol Soc. 2012; 2011:2937-40. DOI: 10.1109/IEMBS.2011.6090808. View

11.

Ostrowski A, Chan E, Gargas D, Katz E, Han G, Schuck P . Controlled synthesis and single-particle imaging of bright, sub-10 nm lanthanide-doped upconverting nanocrystals. ACS Nano. 2012; 6(3):2686-92. DOI: 10.1021/nn3000737. View

12.

Zorzos A, Scholvin J, Boyden E, Fonstad C . Three-dimensional multiwaveguide probe array for light delivery to distributed brain circuits. Opt Lett. 2012; 37(23):4841-3. PMC: 3572236. DOI: 10.1364/OL.37.004841. View

13.

Abaya T, Blair S, Tathireddy P, Rieth L, Solzbacher F . A 3D glass optrode array for optical neural stimulation. Biomed Opt Express. 2012; 3(12):3087-104. PMC: 3521295. DOI: 10.1364/BOE.3.003087. View

14.

Zou C, Shu F, Sun F, Gong Z, Han Z, Guo G . Theory of free space coupling to high-Q whispering gallery modes. Opt Express. 2013; 21(8):9982-95. DOI: 10.1364/OE.21.009982. View

15.

Zhong M, Wei X, Zhou J, Wang Z, Li Y . Trapping red blood cells in living animals using optical tweezers. Nat Commun. 2013; 4:1768. DOI: 10.1038/ncomms2786. View

16.

Wu F, Stark E, Im M, Cho I, Yoon E, Buzsaki G . An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications. J Neural Eng. 2013; 10(5):056012. PMC: 4056669. DOI: 10.1088/1741-2560/10/5/056012. View

17.

Zhao J, Jin D, Schartner E, Lu Y, Liu Y, Zvyagin A . Single-nanocrystal sensitivity achieved by enhanced upconversion luminescence. Nat Nanotechnol. 2013; 8(10):729-34. DOI: 10.1038/nnano.2013.171. View

18.

Horton N, Wang K, Kobat D, Clark C, Wise F, Schaffer C . three-photon microscopy of subcortical structures within an intact mouse brain. Nat Photonics. 2013; 7(3). PMC: 3864872. DOI: 10.1038/nphoton.2012.336. View

19.

Gargas D, Chan E, Ostrowski A, Aloni S, Altoe M, S Barnard E . Engineering bright sub-10-nm upconverting nanocrystals for single-molecule imaging. Nat Nanotechnol. 2014; 9(4):300-5. DOI: 10.1038/nnano.2014.29. View

20.

Pashaie R, Anikeeva P, Lee J, Prakash R, Yizhar O, Prigge M . Optogenetic brain interfaces. IEEE Rev Biomed Eng. 2014; 7:3-30. DOI: 10.1109/RBME.2013.2294796. View