Trapping Red Blood Cells in Living Animals Using Optical Tweezers

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Apr 25

PMID 23612309

Citations 65

Authors

Min-Cheng Zhong

Xun-Bin Wei

Jin-Hua Zhou

Zi-Qiang Wang

Yin-Mei Li

Affiliations

Soon will be listed here.

Abstract

The recent development of non-invasive imaging techniques has enabled the visualization of molecular events underlying cellular processes in live cells. Although microscopic objects can be readily manipulated at the cellular level, additional physiological insight is likely to be gained by manipulation of cells in vivo, which has not been achieved so far. Here we use infrared optical tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice. We realize a non-contact micro-operation that results in the clearing of a blocked microvessel. Furthermore, we estimate the optical trap stiffness in the capillary. Our work expands the application of optical tweezers to the study of live cell dynamics in animals.

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