Novel Classes of Replication-associated Transcripts Discovered in Viruses

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2019 Jan 5

PMID 30608222

Citations 11

Authors

Zsolt Boldogkoi

Zsolt Balazs

Norbert Moldovan

Istvan Prazsak

Dora Tombacz

Affiliations

Soon will be listed here.

Abstract

The role of RNA molecules in the priming of DNA replication and in providing a template for telomerase extension has been known for decades. Since then, several transcripts have been discovered, which play diverse roles in governing replication, including regulation of RNA primer formation, the recruitment of replication origin (Ori) recognition complex, and the assembly of replication fork. Recent studies on viral transcriptomes have revealed novel classes of replication-associated (ra)RNAs, which are expressed from the genomic locations in close vicinity to the Ori. Many of them overlap the Ori, whereas others are terminated close to the replication origin. These novel transcripts can be both protein-coding and non-coding RNAs. The Ori-overlapping part of the mRNAs is generally either the 5'-untranslated regions (UTRs), or the 3'-UTRs of the longer isoforms. Several raRNAs have been identified in various viral families using primarily third-generation long-read sequencing. Hyper-editing of these transcripts has also been described.

Citing Articles

KSHV 3.0: a state-of-the-art annotation of the Kaposi's sarcoma-associated herpesvirus transcriptome using cross-platform sequencing.

Prazsak I, Tombacz D, Fulop A, Torma G, Gulyas G, Dormo A mSystems. 2024; 9(2):e0100723.

PMID: 38206015 PMC: 10878076. DOI: 10.1128/msystems.01007-23.

Identification of herpesvirus transcripts from genomic regions around the replication origins.

Torma G, Tombacz D, Csabai Z, Almsarrhad I, Nagy G, Kakuk B Sci Rep. 2023; 13(1):16395.

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Hybrid sequencing discloses unique aspects of the transcriptomic architecture in equid alphaherpesvirus 1.

Tombacz D, Torma G, Gulyas G, Fulop A, Dormo A, Prazsak I Heliyon. 2023; 9(7):e17716.

PMID: 37449092 PMC: 10336594. DOI: 10.1016/j.heliyon.2023.e17716.

In-Depth Temporal Transcriptome Profiling of an Alphaherpesvirus Using Nanopore Sequencing.

Tombacz D, Kakuk B, Torma G, Csabai Z, Gulyas G, Tamas V Viruses. 2022; 14(6).

PMID: 35746760 PMC: 9229804. DOI: 10.3390/v14061289.

Dual isoform sequencing reveals complex transcriptomic and epitranscriptomic landscapes of a prototype baculovirus.

Torma G, Tombacz D, Moldovan N, Fulop A, Prazsak I, Csabai Z Sci Rep. 2022; 12(1):1291.

PMID: 35079129 PMC: 8789824. DOI: 10.1038/s41598-022-05457-8.

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