Selection of Reference Genes for QRT-PCR Analysis in After Hot-Air Drying

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Jan 4

PMID 30602709

Citations 16

Authors

Shuangshuang Gao

Gangzheng Wang

Zhicheng Huang

Xiaoyu Lei

Yinbing Bian

Ying Liu

Wen Huang

Affiliations

Soon will be listed here.

Abstract

Volatile sulfur compounds gradually develop in after hot-air drying, and many genes are involved in the generation of these sulfur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR). In this study, the expression profile of 17 candidate genes was assessed in under treatment at 50 °C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper. Results indicated that the two optimal reference genes for mycelium and fruiting body were and as well as and , respectively. Additionally, and were found to be the two most stable reference genes across the mycelium and fruiting body set. Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in .

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