Asparagine-linked Glycosylation Modifies Voltage-dependent Gating Properties of Ca3.1-T-type Ca Channel

Overview

Journal J Physiol Sci

Specialty Physiology

Date 2019 Jan 3

PMID 30600443

Citations 7

Authors

Yangong Liu

Pu Wang

Fangfang Ma

Mingqi Zheng

Gang Liu

Shinichiro Kume

Tatsuki Kurokawa

Katsushige Ono

Affiliations

Soon will be listed here.

Abstract

T-type channels are low-voltage-activated channels that play a role in the cardiovascular system particularly for pacemaker activity. Glycosylation is one of the most prevalent post-translational modifications in protein. Among various glycosylation types, the most common one is asparagine-linked (N-linked) glycosylation. The aim of this study was to elucidate the roles of N-linked glycosylation for the gating properties of the Ca3.1-T-type Ca channel. N-linked glycosylation synthesis inhibitor tunicamycin causes a reduction of Ca3.1-T-type Ca channel current (Ca3.1-I) when applied for 12 h or longer. Tunicamycin (24 h) significantly shifted the activation curve to the depolarization potentials, whereas the steady-state inactivation curve was unaffected. Use-dependent inactivation of Ca3.1-I was accelerated, and recovery from inactivation was prolonged by tunicamycin (24 h). Ca3.1-I was insensitive to a glycosidase PNGase F when the channels were expressed on the plasma membrane. These findings suggest that N-glycosylation contributes not only to the cell surface expression of the Ca3.1-T-type Ca channel but to the regulation of the gating properties of the channel when the channel proteins were processed during the folding and trafficking steps in the cell.

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