Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine

Overview

Journal Sci Rep

Specialty Science

Date 2018 Dec 22

PMID 30573748

Citations 20

Authors

David A G Skibinski

Leigh Ann Jones

Yuan O Zhu

Lin Wu Xue

Bijin Au

Bernett Lee

Ahmad Nazri Mohamed Naim

Audrey Lee

Nivashini Kaliaperumal

Jenny G H Low

Lawrence S Lee

Michael Poidinger

Philippe Saudan

Martin Bachmann

Eng Eong Ooi

Brendon J Hanson

Veronica Novotny-Diermayr

Alex Matter

Anna-Marie Fairhurst

Martin L Hibberd

John E Connolly

Affiliations

Soon will be listed here.

Abstract

Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4 and CD8 T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4 T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4 and CD8 T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.

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