Potential CeRNA Networks Involved in Autophagy Suppression of Pancreatic Cancer Caused by Chloroquine Diphosphate: A Study Based on Differentially‑expressed CircRNAs, LncRNAs, MiRNAs and MRNAs

Overview

Journal Int J Oncol

Specialty Oncology

Date 2018 Dec 21

PMID 30570107

Citations 34

Authors

Dan-Ming Wei

Meng-Tong Jiang

Peng Lin

Hong Yang

Yi-Wu Dang

Qiao Yu

Dan-Yu Liao

Dian-Zhong Luo

Gang Chen

Affiliations

Soon will be listed here.

Abstract

Autophagy has been reported to be involved in the occurrence and development of pancreatic cancer. However, the mechanism of autophagy‑associated non‑coding RNAs (ncRNAs) in pancreatic cancer remains largely unknown. In the present study, microarrays were used to detect differential expression of mRNAs, microRNAs (miRNAs), long ncRNAs (lncRNAs) and circular RNAs (circRNAs) post autophagy suppression by chloroquine diphosphate in PANC‑1 cells. Collectively, 3,966 mRNAs, 3,184 lncRNAs and 9,420 circRNAs were differentially expressed. Additionally, only two miRNAs (hsa‑miR‑663a‑5p and hsa‑miR‑154‑3p) were underexpressed in the PANC‑1 cells in the autophagy‑suppression group. Furthermore, miR‑663a‑5p with 9 circRNAs, 8 lncRNAs and 46 genes could form a prospective ceRNA network associated with autophagy in pancreatic cancer cells. In addition, another ceRNA network containing miR‑154‑3p, 5 circRNAs, 2 lncRNAs and 11 genes was also constructed. The potential multiple ceRNA, miRNA and mRNA associations may serve pivotal roles in the autophagy of pancreatic cancer cells, which lays the theoretical foundation for subsequent investigations on pancreatic cancer.

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References

Lovecek M, Skalicky P, Klos D, Bebarova L, Neoral C, Ehrmann J . Long-term survival after resections for pancreatic ductal adenocarcinoma. Single centre study. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016; 160(2):280-6. DOI: 10.5507/bp.2016.011. View

Gafar A, Draz H, Goldberg A, Bashandy M, Bakry S, Khalifa M . Lithocholic acid induces endoplasmic reticulum stress, autophagy and mitochondrial dysfunction in human prostate cancer cells. PeerJ. 2016; 4:e2445. PMC: 5119235. DOI: 10.7717/peerj.2445. View

Chen S, Wu J, Jiao K, Wu Q, Ma J, Chen D . MicroRNA-495-3p inhibits multidrug resistance by modulating autophagy through GRP78/mTOR axis in gastric cancer. Cell Death Dis. 2018; 9(11):1070. PMC: 6195618. DOI: 10.1038/s41419-018-0950-x. View

Krek A, Grun D, Poy M, Wolf R, Rosenberg L, Epstein E . Combinatorial microRNA target predictions. Nat Genet. 2005; 37(5):495-500. DOI: 10.1038/ng1536. View

Klein K, Werner K, Teske C, Schenk M, Giese T, Weitz J . Role of TFEB-driven autophagy regulation in pancreatic cancer treatment. Int J Oncol. 2016; 49(1):164-72. DOI: 10.3892/ijo.2016.3505. View

Liu Y, Ren F, Luo Y, Rong M, Chen G, Dang Y . Down-Regulation of MiR-193a-3p Dictates Deterioration of HCC: A Clinical Real-Time qRT-PCR Study. Med Sci Monit. 2015; 21:2352-60. PMC: 4538786. DOI: 10.12659/MSM.894077. View

Huang W, Li J, Guo X, Zhao Y, Yuan X . miR-663a inhibits hepatocellular carcinoma cell proliferation and invasion by targeting HMGA2. Biomed Pharmacother. 2016; 81:431-438. DOI: 10.1016/j.biopha.2016.04.034. View

Nakata K, Ohuchida K, Mizumoto K, Kayashima T, Ikenaga N, Sakai H . MicroRNA-10b is overexpressed in pancreatic cancer, promotes its invasiveness, and correlates with a poor prognosis. Surgery. 2011; 150(5):916-22. DOI: 10.1016/j.surg.2011.06.017. View

Sionov R, Vlahopoulos S, Granot Z . Regulation of Bim in Health and Disease. Oncotarget. 2015; 6(27):23058-134. PMC: 4695108. DOI: 10.18632/oncotarget.5492. View

10.

Rehmsmeier M, Steffen P, Hochsmann M, Giegerich R . Fast and effective prediction of microRNA/target duplexes. RNA. 2004; 10(10):1507-17. PMC: 1370637. DOI: 10.1261/rna.5248604. View

11.

Zarzynska J . The importance of autophagy regulation in breast cancer development and treatment. Biomed Res Int. 2014; 2014:710345. PMC: 4182068. DOI: 10.1155/2014/710345. View

12.

Georgiadou D, Sergentanis T, Sakellariou S, Vlachodimitropoulos D, Psaltopoulou T, Lazaris A . Prognostic role of sex steroid receptors in pancreatic adenocarcinoma. Pathol Res Pract. 2015; 212(1):38-43. DOI: 10.1016/j.prp.2015.11.007. View

13.

Seo J, Choi J, Lee S, Sung S, Yoo H, Kang M . Autophagy is required for PDAC glutamine metabolism. Sci Rep. 2016; 6:37594. PMC: 5124864. DOI: 10.1038/srep37594. View

14.

Tan Y, Zhang J, Zhou G . Autophagy and its implication in human oral diseases. Autophagy. 2016; 13(2):225-236. PMC: 5324841. DOI: 10.1080/15548627.2016.1234563. View

15.

Ranjan A, Srivastava S . Penfluridol suppresses pancreatic tumor growth by autophagy-mediated apoptosis. Sci Rep. 2016; 6:26165. PMC: 4870635. DOI: 10.1038/srep26165. View

16.

Zhou X, Ye F, Yin C, Zhuang Y, Yue G, Zhang G . The Interaction Between MiR-141 and lncRNA-H19 in Regulating Cell Proliferation and Migration in Gastric Cancer. Cell Physiol Biochem. 2015; 36(4):1440-52. DOI: 10.1159/000430309. View

17.

Tajadini M, Panjehpour M, Javanmard S . Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes. Adv Biomed Res. 2014; 3:85. PMC: 3988599. DOI: 10.4103/2277-9175.127998. View

18.

Massillo C, Dalton G, Farre P, De Luca P, De Siervi A . Implications of microRNA dysregulation in the development of prostate cancer. Reproduction. 2017; 154(4):R81-R97. DOI: 10.1530/REP-17-0322. View

19.

Hua R, Han S, Zhang N, Dai Q, Liu T, Li J . cPKCγ-Modulated Sequential Reactivation of mTOR Inhibited Autophagic Flux in Neurons Exposed to Oxygen Glucose Deprivation/Reperfusion. Int J Mol Sci. 2018; 19(5). PMC: 5983661. DOI: 10.3390/ijms19051380. View

20.

Chen P, Gombart Z, Chen J . Chloroquine treatment of ARPE-19 cells leads to lysosome dilation and intracellular lipid accumulation: possible implications of lysosomal dysfunction in macular degeneration. Cell Biosci. 2011; 1(1):10. PMC: 3125200. DOI: 10.1186/2045-3701-1-10. View