Mechanisms of Circular RNA Circ_0066147 on Pancreatic Cancer Progression

Overview

Journal Open Life Sci

Specialty Biology

Date 2021 May 31

PMID 34056113

Citations 5

Authors

Jie Zhang

Zhang Zhang

Affiliations

Soon will be listed here.

Abstract

Background: The purpose of the study was to explore the precise parts of circ_0066147 (circular RNA [circRNA] scm-like with four mbt domains 1, circSFMBT1) in pancreatic cancer (PC) progression.

Methods: Ribonuclease R assay was used to confirm the stability of circ_0066147. circ_0066147, miR-326 and E2F transcription factor 2 (E2F2) expression levels was detected by quantitative reverse-transcription polymerase chain reaction or Western blot. Cell proliferation, apoptosis, migration and invasion abilities were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2-tetrazolium bromide, flow cytometry, wound-healing and transwell assays, respectively. Targeted relationships among circ_0066147, miR-326 and E2F2 were verified by the dual-luciferase reporter or RNA pull-down assay.

Results: circ_0066147 expression was upregulated in PC tissues and cells. circ_0066147 knockdown inhibited PC cell proliferation, migration, invasion and enhanced apoptosis , as well as weakened tumor growth . Mechanistically, circ_0066147 directly targeted miR-326 and circ_0066147 modulated E2F2 expression by miR-326. miR-326 mediated the regulation of circ_0066147 in PC cell behaviors . Furthermore, E2F2 was a functional target of miR-326 in modulating PC cell behaviors .

Conclusion: circ_0066147 regulated PC malignant progression in part depending on the miR-326/E2F2 axis, illuminating circ_0066147 was a potential prognostic marker and therapeutic target for PC management.

Citing Articles

CircRNAs in Pancreatic Cancer: New Tools for Target Identification and Therapeutic Intervention.

Weidle U, Nopora A Cancer Genomics Proteomics. 2024; 21(4):327-349.

PMID: 38944427 PMC: 11215428. DOI: 10.21873/cgp.20451.

Diagnostic and prognostic role of circRNAs in pancreatic cancer: a meta-analysis.

Zhao R, Han Z, Zhou H, Xue Y, Chen X, Cao X Front Oncol. 2023; 13:1174577.

PMID: 37361594 PMC: 10285410. DOI: 10.3389/fonc.2023.1174577.

Prognostic, Diagnostic, and Clinicopathological Significance of Circular RNAs in Pancreatic Cancer: A Systematic Review and Meta-Analysis.

Li J, Ye Z, Hu X, Hou S, Hang Q Cancers (Basel). 2022; 14(24).

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Roles of circular RNAs in the pathogenesis and treatment of pancreatic cancer.

Seimiya T, Otsuka M, Fujishiro M Front Cell Dev Biol. 2022; 10:1023332.

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Identification of a dysregulated CircRNA-associated gene signature for predicting prognosis, immune landscape, and drug candidates in bladder cancer.

Shen C, Li Z, Zhang Y, Zhang Z, Wu Z, Da L Front Oncol. 2022; 12:1018285.

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