Genetically Encoded RNA-based Sensors for Intracellular Imaging of Silver Ions

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2018 Dec 20

PMID 30566125

Citations 11

Authors

Qikun Yu

Jing Shi

Aruni P K K Karunanayake Mudiyanselage

Rigumula Wu

Bin Zhao

Ming Zhou

Mingxu You

Affiliations

Soon will be listed here.

Abstract

Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag+ in live bacterial cells. The sensor is designed by introducing a cytosine-Ag+-cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag+ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag+.

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