Genetically Encoded Ratiometric RNA-Based Sensors for Quantitative Imaging of Small Molecules in Living Cells

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2019 Oct 9

PMID 31591798

Citations 21

Authors

Rigumula Wu

Aruni P K K Karunanayake Mudiyanselage

Fatemeh Shafiei

Bin Zhao

Yousef Bagheri

Qikun Yu

Kathleen McAuliffe

Kewei Ren

Mingxu You

Affiliations

Soon will be listed here.

Abstract

Precisely determining the intracellular concentrations of metabolites and signaling molecules is critical in studying cell biology. Fluorogenic RNA-based sensors have emerged to detect various targets in living cells. However, it is still challenging to apply these genetically encoded sensors to quantify the cellular concentrations and distributions of targets. Herein, using a pair of orthogonal fluorogenic RNA aptamers, DNB and Broccoli, we engineered a modular sensor system to apply the DNB-to-Broccoli fluorescence ratio to quantify the cell-to-cell variations of target concentrations. These ratiometric sensors can be broadly applied for live-cell imaging and quantification of metabolites, signaling molecules, and other synthetic compounds.

Citing Articles

Near-infrared fluorogenic RNA for in vivo imaging and sensing.

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Multiplexed sequential imaging in living cells with orthogonal fluorogenic RNA aptamer/dye pairs.

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