The Lack of Slow Force Response in Failing Rat Myocardium: Role of Stretch-induced Modulation of Ca-TnC Kinetics

Overview

Journal J Physiol Sci

Specialty Physiology

Date 2018 Dec 19

PMID 30560346

Citations 5

Authors

Oleg Lookin

Yuri Protsenko

Affiliations

Soon will be listed here.

Abstract

The slow force response (SFR) to stretch is an important adaptive mechanism of the heart. The SFR may result in ~ 20-30% extra force but it is substantially attenuated in heart failure. We investigated the relation of SFR magnitude with Ca transient decay in healthy (CONT) and monocrotaline-treated rats with heart failure (MCT). Right ventricular trabeculae were stretched from 85 to 95% of optimal length and held stretched for 10 min at 30 °C and 1 Hz. Isometric twitches and Ca transients were collected on 2, 4, 6, 8, 10 min after stretch. The changes in peak tension and Ca transient decay characteristics during SFR were evaluated as a percentage of the value measured immediately after stretch. The amount of Ca utilized by TnC was indirectly evaluated using the methods of Ca transient "bump" and "difference curve." The muscles of CONT rats produced positive SFR and they showed prominent functional relation between SFR magnitude and the magnitude (amplitude, integral intensity) of Ca transient "bump" and "difference curve." The myocardium of MCT rats showed negative SFR to stretch (force decreased in time) which was not correlated well with the characteristics of Ca transient decay, evaluated by the methods of "bump" and "difference curve." We conclude that the intracellular mechanisms of Ca balancing during stretch-induced slow adaptation of myocardial contractility are disrupted in failing rat myocardium. The potential significance of our findings is that the deficiency of slow force response in diseased myocardium may be diminished under augmented kinetics of Ca-TnC interaction.

Citing Articles

Differences in Effects of Length-Dependent Regulation of Force and Ca Transient in the Myocardial Trabeculae of the Rat Right Atrium and Ventricle.

Lookin O, Balakin A, Protsenko Y Int J Mol Sci. 2023; 24(10).

PMID: 37240302 PMC: 10219166. DOI: 10.3390/ijms24108960.

Contractile Behavior of Right Atrial Myocardium of Healthy Rats and Rats with the Experimental Model of Pulmonary Hypertension.

Lookin O, Mukhlynina E, Protsenko Y Int J Mol Sci. 2022; 23(8).

PMID: 35457003 PMC: 9029478. DOI: 10.3390/ijms23084186.

Mitogen-activated protein kinase p38 modulates pacemaker ion channels differentiation in P19-derived pluripotent cells.

Zheng M, Kang L, Uchino T, Liu G, Wang Y, Ono K J Physiol Sci. 2020; 70(1):39.

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Cardiac specific transcription factor Csx/Nkx2.5 regulates transient-outward K channel expression in pluripotent P19 cell-derived cardiomyocytes.

Uchino T, Zheng M, Wang Y, Ono K J Physiol Sci. 2020; 70(1):20.

PMID: 32213161 PMC: 7096375. DOI: 10.1186/s12576-020-00748-z.

Length-Dependent Activation of Contractility and Ca-Transient Kinetics in Auxotonically Contracting Isolated Rat Ventricular Cardiomyocytes.

Lookin O, Protsenko Y Front Physiol. 2020; 10:1473.

PMID: 31920687 PMC: 6917588. DOI: 10.3389/fphys.2019.01473.

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