Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 Dec 15

PMID 30544709

Citations 2

Authors

Mi-Kyoung Kim

Hyun-Joo Park

Yeon Kim

Soo-Kyung Bae

Hyung Joon Kim

Moon-Kyoung Bae

Affiliations

Soon will be listed here.

Abstract

Gastrin-releasing peptide (GRP), a member of bombesin-like peptides, and its receptor (GRP-R) play an important role in various physiological and pathological conditions. In this work, we investigated the role of GRP-R on adipogenesis in 3T3-L1 adipocytes. The expression of GRP-R was significantly increased during the adipocyte differentiation of 3T3-L1 cells. The inhibition of GRP-R by the antagonist RC-3095 affected adipogenesis in 3T3-L1 cells, which reduced lipid accumulation and regulated the expression of adipogenic genes. Moreover, cyclic AMP response element-binding protein (CREB) directly bound to the GRP-R promoter upon exposure to adipogenic stimuli. The down-regulation of GRP-R by the knockdown of CREB inhibited adipocyte differentiation of 3T3-L1 cells. Together these results suggest that the regulation of GRP-R activity or expression has an influence on adipogenesis through regulating adipogenic related genes.

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References

Reusch J, Colton L, Klemm D . CREB activation induces adipogenesis in 3T3-L1 cells. Mol Cell Biol. 2000; 20(3):1008-20. PMC: 85218. DOI: 10.1128/MCB.20.3.1008-1020.2000. View

Weber H, Jensen R, Battey J . Molecular organization of the mouse gastrin-releasing peptide receptor gene and its promoter. Gene. 2000; 244(1-2):137-49. DOI: 10.1016/s0378-1119(99)00563-6. View

Xiao D, Wang J, Hampton L, Weber H . The human gastrin-releasing peptide receptor gene structure, its tissue expression and promoter. Gene. 2001; 264(1):95-103. DOI: 10.1016/s0378-1119(00)00596-5. View

Yang Y, Song H, Li R, Zhou L, Zhu Z, Hu R . The gene expression profiling of human visceral adipose tissue and its secretory functions. Biochem Biophys Res Commun. 2003; 300(4):839-46. DOI: 10.1016/s0006-291x(02)02843-7. View

Zhang J, Klemm D, Vinson C, Lane M . Role of CREB in transcriptional regulation of CCAAT/enhancer-binding protein beta gene during adipogenesis. J Biol Chem. 2003; 279(6):4471-8. DOI: 10.1074/jbc.M311327200. View

Qu X, Xiao D, Weber H . Transcriptional activation of the human gastrin-releasing peptide receptor gene in gastrointestinal and prostatic epithelial cancer cells. J Mol Neurosci. 2004; 22(1-2):75-82. DOI: 10.1385/JMN:22:1-2:75. View

Tilg H, Moschen A . Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol. 2006; 6(10):772-83. DOI: 10.1038/nri1937. View

Farmer S . Transcriptional control of adipocyte formation. Cell Metab. 2006; 4(4):263-73. PMC: 1958996. DOI: 10.1016/j.cmet.2006.07.001. View

Hoggard N, Bashir S, Cruickshank M, Miller J, Speakman J . Expression of neuromedin B in adipose tissue and its regulation by changes in energy balance. J Mol Endocrinol. 2007; 39(3):199-210. DOI: 10.1677/JME-07-0071. View

10.

Lago F, Dieguez C, Gomez-Reino J, Gualillo O . Adipokines as emerging mediators of immune response and inflammation. Nat Clin Pract Rheumatol. 2007; 3(12):716-24. DOI: 10.1038/ncprheum0674. View

11.

Gonzalez N, Moody T, Igarashi H, Ito T, Jensen R . Bombesin-related peptides and their receptors: recent advances in their role in physiology and disease states. Curr Opin Endocrinol Diabetes Obes. 2008; 15(1):58-64. PMC: 2631407. DOI: 10.1097/MED.0b013e3282f3709b. View

12.

Chinnappan D, Qu X, Xiao D, Ratnasari A, Weber H . Human gastrin-releasing peptide receptor gene regulation requires transcription factor binding at two distinct CRE sites. Am J Physiol Gastrointest Liver Physiol. 2008; 295(1):G153-G162. PMC: 2494719. DOI: 10.1152/ajpgi.00036.2008. View

13.

Weber H . Regulation and signaling of human bombesin receptors and their biological effects. Curr Opin Endocrinol Diabetes Obes. 2009; 16(1):66-71. DOI: 10.1097/med.0b013e32831cf5aa. View

14.

Ischia J, Patel O, Shulkes A, Baldwin G . Gastrin-releasing peptide: different forms, different functions. Biofactors. 2009; 35(1):69-75. DOI: 10.1002/biof.10. View

15.

Rajsheker S, Manka D, Blomkalns A, Chatterjee T, Stoll L, Weintraub N . Crosstalk between perivascular adipose tissue and blood vessels. Curr Opin Pharmacol. 2010; 10(2):191-6. PMC: 2843777. DOI: 10.1016/j.coph.2009.11.005. View

16.

Li X, Lv Y, Yuan A, Li Z . Gastrin-releasing peptide links stressor to cancer progression. J Cancer Res Clin Oncol. 2010; 136(4):483-91. DOI: 10.1007/s00432-010-0766-2. View

17.

Lee M, Wu Y, Fried S . Adipose tissue remodeling in pathophysiology of obesity. Curr Opin Clin Nutr Metab Care. 2010; 13(4):371-6. PMC: 3235038. DOI: 10.1097/MCO.0b013e32833aabef. View

18.

Majumdar I, Weber H . Biology of mammalian bombesin-like peptides and their receptors. Curr Opin Endocrinol Diabetes Obes. 2010; 18(1):68-74. DOI: 10.1097/MED.0b013e328340ff93. View

19.

Szasz T, Webb R . Perivascular adipose tissue: more than just structural support. Clin Sci (Lond). 2011; 122(1):1-12. PMC: 3966487. DOI: 10.1042/CS20110151. View

20.

Oliveira P, Grespan R, Pinto L, Meurer L, Brenol J, Roesler R . Protective effect of RC-3095, an antagonist of the gastrin-releasing peptide receptor, in experimental arthritis. Arthritis Rheum. 2011; 63(10):2956-65. DOI: 10.1002/art.30486. View