Screening of Streptococcus Suis Serotype 2 Resistance Genes with GWAS and Transcriptomic Microarray Analysis

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2018 Dec 14

PMID 30541452

Citations 3

Authors

Zhe Ma

Haodan Zhu

Yiqi Su

Yu Meng

Huixing Lin

Kongwang He

Hongjie Fan

Affiliations

Soon will be listed here.

Abstract

Background: Swine streptococcosis has caused great economic loss in the swine industry, and the major pathogen responsible for this disease is Streptococcus Suis serotype 2 (SS2). Disease resistance breeding is a fundamental way of resolving this problem. With the development of GWAS and transcriptomic microarray technology, we now have powerful research tools to identify SS2 resistance genes.

Results: In this research, we generated an F generation of SS2 resistant C57BL/6 and SS2 susceptive A/J mice. With the F generation of these two mice strains and GWAS analysis, we identified 286 significant mouse genome SNPs sites associated with the SS2 resistance trait. Gene expression profiles for C57BL/6 and A/J were analyzed under SS2 infection pressure by microarray. In total, 251 differentially expressed genes were identified between these two mouse strains during SS2 infection. After combining the GWAS and gene expression profile data, we located two genes that were significantly associated with SS2 resistance, which were the UBA domain containing 1 gene (Ubac1) and Epsin 1 gene (Epn 1). GO classification and over-representation analysis revealed nine up-regulated related to immune function, which could potentially be involved in the C57BL/6 SS2 resistance trait.

Conclusion: This is the first study to use both SNP chip and gene express profile chip for SS2 resistance gene identification in mouse, and these results will contribute to swine SS2 resistance breeding.

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