A Virus-packageable CRISPR Screen Identifies Host Factors Mediating Interferon Inhibition of HIV

Overview

Journal Elife

Specialty Biology

Date 2018 Dec 7

PMID 30520725

Citations 87

Authors

Molly Ohainle

Louisa Helms

Jolien Vermeire

Ferdinand Roesch

Daryl Humes

Ryan Basom

Jeffrey J Delrow

Julie Overbaugh

Michael Emerman

Affiliations

Soon will be listed here.

Abstract

Editorial Note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).

Citing Articles

Comprehensive maps of escape mutations from antibodies 10-1074 and 3BNC117 for Envs from two divergent HIV strains.

Radford C, Bloom J bioRxiv. 2025; .

PMID: 39975072 PMC: 11838258. DOI: 10.1101/2025.01.30.635715.

Cell-free assays reveal that the HIV-1 capsid protects reverse transcripts from cGAS immune sensing.

Scott T, Arnold L, Powers J, McCann D, Rowe A, Christensen D PLoS Pathog. 2025; 21(1):e1012206.

PMID: 39874383 PMC: 11793794. DOI: 10.1371/journal.ppat.1012206.

Cyclophilin A Regulates Tripartite Motif 5 Alpha Restriction of HIV-1.

Wang T, Becker D, Twizerimana A, Luedde T, Gohlke H, Munk C Int J Mol Sci. 2025; 26(2).

PMID: 39859212 PMC: 11764967. DOI: 10.3390/ijms26020495.

Functionally-informed fine-mapping identifies genetic variants linking increased CHD1L expression and HIV restriction in monocytes.

Tough R, McLaren P Sci Rep. 2025; 15(1):2325.

PMID: 39825011 PMC: 11748618. DOI: 10.1038/s41598-024-84817-y.

Functional anatomy of zinc finger antiviral protein complexes.

Bohn J, Meagher J, Takata M, Goncalves-Carneiro D, Yeoh Z, Ohi M Nat Commun. 2024; 15(1):10834.

PMID: 39738020 PMC: 11685948. DOI: 10.1038/s41467-024-55192-z.

References

Apps R, Del Prete G, Chatterjee P, Lara A, Brumme Z, Brockman M . HIV-1 Vpu Mediates HLA-C Downregulation. Cell Host Microbe. 2016; 19(5):686-95. PMC: 4904791. DOI: 10.1016/j.chom.2016.04.005. View

Anders S, Pyl P, Huber W . HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics. 2014; 31(2):166-9. PMC: 4287950. DOI: 10.1093/bioinformatics/btu638. View

Fenton-May A, Dibben O, Emmerich T, Ding H, Pfafferott K, Aasa-Chapman M . Relative resistance of HIV-1 founder viruses to control by interferon-alpha. Retrovirology. 2013; 10:146. PMC: 3907080. DOI: 10.1186/1742-4690-10-146. View

Schoggins J, Wilson S, Panis M, Murphy M, Jones C, Bieniasz P . A diverse range of gene products are effectors of the type I interferon antiviral response. Nature. 2011; 472(7344):481-5. PMC: 3409588. DOI: 10.1038/nature09907. View

Van Damme N, Goff D, Katsura C, Jorgenson R, Mitchell R, Johnson M . The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein. Cell Host Microbe. 2008; 3(4):245-52. PMC: 2474773. DOI: 10.1016/j.chom.2008.03.001. View

Sheehy A, Gaddis N, Choi J, Malim M . Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature. 2002; 418(6898):646-50. DOI: 10.1038/nature00939. View

Bick M, Carroll J, Gao G, Goff S, Rice C, MacDonald M . Expression of the zinc-finger antiviral protein inhibits alphavirus replication. J Virol. 2003; 77(21):11555-62. PMC: 229374. DOI: 10.1128/jvi.77.21.11555-11562.2003. View

Jolly C, Booth N, Neil S . Cell-cell spread of human immunodeficiency virus type 1 overcomes tetherin/BST-2-mediated restriction in T cells. J Virol. 2010; 84(23):12185-99. PMC: 2976402. DOI: 10.1128/JVI.01447-10. View

Goujon C, Moncorge O, Bauby H, Doyle T, Ward C, Schaller T . Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection. Nature. 2013; 502(7472):559-62. PMC: 3808269. DOI: 10.1038/nature12542. View

10.

Gummuluru S, Kinsey C, Emerman M . An in vitro rapid-turnover assay for human immunodeficiency virus type 1 replication selects for cell-to-cell spread of virus. J Virol. 2000; 74(23):10882-91. PMC: 113167. DOI: 10.1128/jvi.74.23.10882-10891.2000. View

11.

Rosa A, Chande A, Ziglio S, De Sanctis V, Bertorelli R, Goh S . HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation. Nature. 2015; 526(7572):212-7. PMC: 4861059. DOI: 10.1038/nature15399. View

12.

Kirmaier A, Wu F, Newman R, Hall L, Morgan J, OConnor S . TRIM5 suppresses cross-species transmission of a primate immunodeficiency virus and selects for emergence of resistant variants in the new species. PLoS Biol. 2010; 8(8). PMC: 2927514. DOI: 10.1371/journal.pbio.1000462. View

13.

Liberatore R, Mastrocola E, Powell C, Bieniasz P . Tetherin Inhibits Cell-Free Virus Dissemination and Retards Murine Leukemia Virus Pathogenesis. J Virol. 2017; 91(12). PMC: 5446635. DOI: 10.1128/JVI.02286-16. View

14.

Lu J, Pan Q, Rong L, He W, Liu S, Liang C . The IFITM proteins inhibit HIV-1 infection. J Virol. 2010; 85(5):2126-37. PMC: 3067758. DOI: 10.1128/JVI.01531-10. View

15.

Li J, Ding S, Cho H, Chung B, Gale Jr M, Chanda S . A short hairpin RNA screen of interferon-stimulated genes identifies a novel negative regulator of the cellular antiviral response. mBio. 2013; 4(3):e00385-13. PMC: 3684836. DOI: 10.1128/mBio.00385-13. View

16.

Langmead B, Trapnell C, Pop M, Salzberg S . Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009; 10(3):R25. PMC: 2690996. DOI: 10.1186/gb-2009-10-3-r25. View

17.

Opp S, Vieira D, Schulte B, Chanda S, Diaz-Griffero F . MxB Is Not Responsible for the Blocking of HIV-1 Infection Observed in Alpha Interferon-Treated Cells. J Virol. 2016; 90(6):3056-64. PMC: 4810631. DOI: 10.1128/JVI.03146-15. View

18.

Lim E, Malik H, Emerman M . Ancient adaptive evolution of tetherin shaped the functions of Vpu and Nef in human immunodeficiency virus and primate lentiviruses. J Virol. 2010; 84(14):7124-34. PMC: 2898239. DOI: 10.1128/JVI.00468-10. View

19.

Lodermeyer V, Suhr K, Schrott N, Kolbe C, Sturzel C, Krnavek D . 90K, an interferon-stimulated gene product, reduces the infectivity of HIV-1. Retrovirology. 2013; 10:111. PMC: 3827937. DOI: 10.1186/1742-4690-10-111. View

20.

Duggal N, Emerman M . Evolutionary conflicts between viruses and restriction factors shape immunity. Nat Rev Immunol. 2012; 12(10):687-95. PMC: 3690816. DOI: 10.1038/nri3295. View