Chemical Analysis of in Vivo-irradiated Dentine of Head and Neck Cancer Patients by ATR-FTIR and Raman Spectroscopy

Overview

Journal Clin Oral Investig

Specialty Dentistry

Date 2018 Dec 6

PMID 30515577

Citations 11

Authors

Rafael Resende de Miranda

Anielle Christine Almeida Silva

Noelio Oliveira Dantas

Carlos Jose Soares

Veridiana Resende Novais

Affiliations

Soon will be listed here.

Abstract

Objectives: To evaluate the effect of in vivo radiotherapy on the chemical properties of human dentine by Fourier-transform infrared spectroscopy (FTIR) and Raman analysis.

Materials And Methods: Chemical composition was evaluated comparing control and irradiated group (n = 8). Irradiated teeth were obtained from radiotherapy patients subjected to fractionated X-ray radiation of 1.8 Gy daily totaling 72 Gy. The teeth were sectioned according to the type of dentine (crown or root dentine), obtaining 3-mm dentine cervical slices. The analyzed parameters by FTIR and Raman spectroscopies were mineral/matrix ratio (M:M), carbonate/mineral ratio (C:M), amide I/amide III ratio, and amide I/CH ratio. Raman also calculated the phosphate and carbonate crystallinity.

Results: FTIR revealed that M:M had a decrease in both factors (p = 0.008; p = 0.043, respectively) and root dentine showed a lower C:M in the irradiated group (p = 0.003). Raman revealed a higher phosphate crystallinity and a lower carbonate crystallinity in crown dentine of irradiated group (p = 0.021; p = 0.039). For amide I/amide III, the irradiated showed a lower ratio when compared to the control group (FTIR p = 0.002; Raman p = 0.017). For amide I/CH, the root dentine showed a higher ratio than the crown dentine in both methods (p < 0.001).

Conclusions: Radiotherapy altered the chemical composition of human dentine. The exchange of phosphate-carbonate ions in the hydroxyapatite and higher concentration of organic components was found after radiotherapy.

Clinical Relevance: The increased risk of radiation-related caries in patients undergoing head and neck radiotherapy is due not only to salivary, dietary, and microbiological changes but also to changes in tooth chemical composition.

Citing Articles

Effect of Periodontal Instrumentation on Tooth Structure Exposure to Ionizing Radiation: In Vitro Study.

Cruz G, Alves R, Orsi C, Faria-E-Silva A, Pigossi S, Soares P Braz Dent J. 2024; 35:e245763.

PMID: 39476046 PMC: 11520504. DOI: 10.1590/0103-6440202405763.

Influence of irradiated dentin, biofilm and different artificial saliva formulations on root dentin demineralization.

de Souza B, Braga A, Vertuan M, Sassaki S, Araujo T, Santos P Heliyon. 2024; 10(16):e36334.

PMID: 39247326 PMC: 11378960. DOI: 10.1016/j.heliyon.2024.e36334.

Effect of radiotherapy, immediate dentin sealing and irrigation simulating single- or two-visits endodontic treatment on the bond strength to pulp chamber dentin: an in vitro study.

Pauletto G, Machry R, Baumhardt T, de Freitas Daudt N, Pereira G, Bier C Odontology. 2024; 113(1):261-273.

PMID: 38951300 DOI: 10.1007/s10266-024-00971-9.

Are Discrepancies in the Amount of Radiation Delivered to Target Neoplastic Cells and Teeth in Cases of Head and Neck Cancer Significant Considerations in Laboratory Research?.

Garcia L, Ribeiro L Eur J Dent. 2024; 18(2):415-416.

PMID: 38806046 PMC: 11132768. DOI: 10.1055/s-0044-1785189.

Assessment of multispecies biofilm growth on root canal dentin under different radiation therapy regimens.

Goulart T, Hawerroth T, Teixeira C, Cesca K, Silva R, de Moraes R Clin Oral Investig. 2024; 28(6):324.

PMID: 38761225 DOI: 10.1007/s00784-024-05719-x.

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