Improved Performance of Lateral Flow Immunoassays for Alpha-fetoprotein and Vanillin by Using Silica Shell-stabilized Gold Nanoparticles

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2018 Dec 6

PMID 30515570

Citations 14

Authors

Xuewen Lu

Ting Mei

Qi Guo

Wenjing Zhou

Xiaomei Li

Jitao Chen

Xinke Zhou

Ning Sun

Zhiyuan Fang

Affiliations

Soon will be listed here.

Abstract

The sensitivity of lateral flow assays (LFA) was increased 30-fold by making use of spherical core-shell gold-silica nanoparticles (AuNP@SiO NPs). They can be prepared by silylation of surfactant stabilized AuNPs. The AuNP@SiO NPs are highly stable and can be used to label antibodies at virtually any concentration. The detection limit of an LFA for alpha-fetoprotein (AFP) can be decreased from 10 ng·mL to 300 pg·mL which makes it comparable to an enzyme-linked immunosorbent assay. To demonstrate the applicability to an immunoassay, a sandwich assay was developed for vanillin by covalent modification of the AuNP@SiO NPs with antibody. By using the method, vanillin can be detected visually in milk powder samples in concentrations as low as 100 ng·g. With unique optical property and great stability, this AuNP@SiO endows great potential in biosensing applications. Graphical abstract Controlled growth of AuNP@SiO. The newly prepared AuNP has a negative hydration layer. This layer is further surrounded by a bilayer of CTAB through electrostatic attraction. The hydrophobic inner layer enables the access and assembling of APTES and MTTS. After the hydrolysis of siloxane, a thin layer of silica shell is formed around AuNP.

Citing Articles

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References

Zhu X, Wu L, Mungra D, Xia S, Zhu J . Au@SiO2 core-shell nanoparticles for laser desorption/ionization time of flight mass spectrometry. Analyst. 2012; 137(10):2454-8. DOI: 10.1039/c2an35074b. View

Huang L, Hou K, Jia X, Pan H, Du M . Preparation of novel silver nanoplates/graphene composite and their application in vanillin electrochemical detection. Mater Sci Eng C Mater Biol Appl. 2014; 38:39-45. DOI: 10.1016/j.msec.2014.01.037. View

Gautier J, Allard-Vannier E, Herve-Aubert K, Souce M, Chourpa I . Design strategies of hybrid metallic nanoparticles for theragnostic applications. Nanotechnology. 2013; 24(43):432002. DOI: 10.1088/0957-4484/24/43/432002. View

Gambinossi F, Mylon S, Ferri J . Aggregation kinetics and colloidal stability of functionalized nanoparticles. Adv Colloid Interface Sci. 2014; 222:332-49. DOI: 10.1016/j.cis.2014.07.015. View

Song J, Zhang X, Qin M, Zhao Y . One-pot two-step synthesis of core-shell mesoporous silica-coated gold nanoparticles. Dalton Trans. 2015; 44(17):7752-6. DOI: 10.1039/c5dt00912j. View

Chu Z, Yin C, Zhang S, Lin G, Li Q . Surface plasmon enhanced drug efficacy using core-shell Au@SiO2 nanoparticle carrier. Nanoscale. 2013; 5(8):3406-11. DOI: 10.1039/c3nr00040k. View

Nie L, Liu F, Ma P, Xiao X . Applications of gold nanoparticles in optical biosensors. J Biomed Nanotechnol. 2015; 10(10):2700-21. DOI: 10.1166/jbn.2014.1987. View

Liu M, Wang Z, Zong S, Chen H, Zhu D, Wu L . SERS detection and removal of mercury(II)/silver(I) using oligonucleotide-functionalized core/shell magnetic silica sphere@Au nanoparticles. ACS Appl Mater Interfaces. 2014; 6(10):7371-9. DOI: 10.1021/am5006282. View

Kharisov B, Kharissova O, Yacaman M, Ortiz M U . State of the art of the bi- and trimetallic nanoparticles on the basis of gold and iron. Recent Pat Nanotechnol. 2009; 3(2):81-98. DOI: 10.2174/187221009788490031. View

10.

Zhang X . Gold Nanoparticles: Recent Advances in the Biomedical Applications. Cell Biochem Biophys. 2015; 72(3):771-5. DOI: 10.1007/s12013-015-0529-4. View

11.

Wu J, Yang Z, Chen N, Zhu W, Hong J, Huang C . Vanillin-molecularly targeted extraction of stir bar based on magnetic field induced self-assembly of multifunctional Fe3O4@Polyaniline nanoparticles for detection of vanilla-flavor enhancers in infant milk powders. J Colloid Interface Sci. 2014; 442:22-9. DOI: 10.1016/j.jcis.2014.11.025. View

12.

Goodner K, Jella P, Rouseff R . Determination of vanillin in orange, grapefruit, tangerine, lemon, and lime juices using GC-olfactometry and GC-MS/MS. J Agric Food Chem. 2000; 48(7):2882-6. DOI: 10.1021/jf990561d. View

13.

Liu W, Zhu Z, Deng K, Li Z, Zhou Y, Qiu H . Gold nanorod@chiral mesoporous silica core-shell nanoparticles with unique optical properties. J Am Chem Soc. 2013; 135(26):9659-64. DOI: 10.1021/ja312327m. View

14.

Timotheou-Potamia M, Calokerinos A . Chemiluminometric determination of vanillin in commercial vanillin products. Talanta. 2008; 71(1):208-12. DOI: 10.1016/j.talanta.2006.03.046. View

15.

Li W, Liao P, Su C, Yeh C . Formation of oligonucleotide-gated silica shell-coated Fe₃O₄-Au core-shell nanotrisoctahedra for magnetically targeted and near-infrared light-responsive theranostic platform. J Am Chem Soc. 2014; 136(28):10062-75. DOI: 10.1021/ja504118q. View

16.

Liu G, Li Q, Ni W, Zhang N, Zheng X, Wang Y . Cytotoxicity of various types of gold-mesoporous silica nanoparticles in human breast cancer cells. Int J Nanomedicine. 2015; 10:6075-87. PMC: 4598223. DOI: 10.2147/IJN.S90887. View

17.

Shi Q, Huang J, Sun Y, Deng R, Teng M, Li Q . A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay. Mikrochim Acta. 2018; 185(2):84. DOI: 10.1007/s00604-017-2556-x. View

18.

Minematsu S, Xuan G, Wu X . Determination of vanillin in vanilla perfumes and air by capillary electrophoresis. J Environ Sci (China). 2014; 25 Suppl 1:S8-S14. DOI: 10.1016/S1001-0742(14)60617-3. View

19.

Liu A, Ye B . Application of gold nanoparticles in biomedical researches and diagnosis. Clin Lab. 2013; 59(1-2):23-36. View

20.

Bononi M, Quaglia G, Tateo F . Easy Extraction Method To Evaluate δ13C Vanillin by Liquid Chromatography-Isotopic Ratio Mass Spectrometry in Chocolate Bars and Chocolate Snack Foods. J Agric Food Chem. 2015; 63(19):4777-81. DOI: 10.1021/acs.jafc.5b02136. View