Karyotype and Chromosomal Characteristics of RDNA of Karaman, 1955 (Teleostei, Cobitidae) from Lake Volvi, Greece

Overview

Journal Comp Cytogenet

Date 2018 Dec 1

PMID 30498563

Citations 1

Authors

Eva Hnatkova

Costas Triantaphyllidis

Catherine Ozouf-Costaz

Lukas Choleva

Zuzana Majtanova

Joerg Bohlen

Petr Rab

Affiliations

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Abstract

The karyotype of Greek cobitid fish Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA fluorescence banding and also by hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Băcescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Linnaeus, 1758 are further discussed.

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