Long Noncoding RNA OIP5-AS1 Targets Wnt-7b to Affect Glioma Progression Via Modulation of MiR-410

Overview

Journal Biosci Rep

Specialty Cell Biology

Date 2018 Dec 1

PMID 30498093

Citations 25

Authors

Wei-Li Sun

Tian Kang

Yuan-Yu Wang

Jian-Ping Sun

Chen Li

Hong-Jiang Liu

Yue Yang

Bao-Hua Jiao

Affiliations

Soon will be listed here.

Abstract

The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. , OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.

Citing Articles

A pan-cancer analysis of Wnt family member 7B in human cancers.

Wang R, Wu N, Wang L, Zhang Z, Wang C, Wang Y Cancer Innov. 2024; 3(5):e139.

PMID: 39257440 PMC: 11386237. DOI: 10.1002/cai2.139.

Nanoparticles transfected with plasmid-encoded lncRNA-OIP5-AS1 inhibit renal ischemia-reperfusion injury in mice via the miR-410-3p/Nrf2 axis.

Zhang R, Zhang X, Zhu X, Li T, Li Y, Zhang P Ren Fail. 2024; 46(1):2319327.

PMID: 38419565 PMC: 10906121. DOI: 10.1080/0886022X.2024.2319327.

Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p.

Gu J, Ye Y, Sunil R, Zhan W, Yu R Exp Ther Med. 2023; 26(5):503.

PMID: 37822583 PMC: 10562957. DOI: 10.3892/etm.2023.12202.

Long non-coding RNAs modulate tumor microenvironment to promote metastasis: novel avenue for therapeutic intervention.

Baba S, Baba S, Mir R, Elfaki I, Algehainy N, Ullah M Front Cell Dev Biol. 2023; 11:1164301.

PMID: 37384249 PMC: 10299194. DOI: 10.3389/fcell.2023.1164301.

Integrative analysis of expression profile indicates the ECM receptor and LTP dysfunction in the glioma-related epilepsy.

Wang Z, Qu J, Xie P, Yang Z, Mao C, Zhang Y BMC Genomics. 2022; 23(1):430.

PMID: 35676651 PMC: 9175475. DOI: 10.1186/s12864-022-08665-8.

References

Zhang T, Wang Y, Zeng F, Cao H, Zhou H, Wang Y . LncRNA H19 is overexpressed in glioma tissue, is negatively associated with patient survival, and promotes tumor growth through its derivative miR-675. Eur Rev Med Pharmacol Sci. 2016; 20(23):4891-4897. View

Verhaak R, Hoadley K, Purdom E, Wang V, Qi Y, Wilkerson M . Integrated genomic analysis identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR, and NF1. Cancer Cell. 2010; 17(1):98-110. PMC: 2818769. DOI: 10.1016/j.ccr.2009.12.020. View

Carro M, Lim W, Alvarez M, Bollo R, Zhao X, Snyder E . The transcriptional network for mesenchymal transformation of brain tumours. Nature. 2009; 463(7279):318-25. PMC: 4011561. DOI: 10.1038/nature08712. View

Liu X, Zheng J, Xue Y, Yu H, Gong W, Wang P . PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop regulates the biological behavior of glioma cells. Theranostics. 2018; 8(4):1084-1105. PMC: 5817112. DOI: 10.7150/thno.21740. View

Hwang J, Smith C, Salhia B, Rutka J . The role of fascin in the migration and invasiveness of malignant glioma cells. Neoplasia. 2008; 10(2):149-59. PMC: 2244690. DOI: 10.1593/neo.07909. View

Yang N, Chen J, Zhang H, Wang X, Yao H, Peng Y . LncRNA OIP5-AS1 loss-induced microRNA-410 accumulation regulates cell proliferation and apoptosis by targeting KLF10 via activating PTEN/PI3K/AKT pathway in multiple myeloma. Cell Death Dis. 2017; 8(8):e2975. PMC: 5596549. DOI: 10.1038/cddis.2017.358. View

Argyriou A, Kalofonos H . Molecularly targeted therapies for malignant gliomas. Mol Med. 2009; 15(3-4):115-22. PMC: 2646824. DOI: 10.2119/molmed.2008.00123. View

Deng J, Deng H, Liu C, Liang Y, Wang S . Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother. 2017; 98:102-110. DOI: 10.1016/j.biopha.2017.12.031. View

Liu X, Wang L, Zhao S, Ji X, Luo Y, Ling F . β-Catenin overexpression in malignant glioma and its role in proliferation and apoptosis in glioblastma cells. Med Oncol. 2010; 28(2):608-14. DOI: 10.1007/s12032-010-9476-5. View

10.

Sun G, Shi L, Yan S, Wan Z, Jiang N, Fu L . MiR-15b targets cyclin D1 to regulate proliferation and apoptosis in glioma cells. Biomed Res Int. 2014; 2014:687826. PMC: 4065679. DOI: 10.1155/2014/687826. View

11.

Bayne K . Revised Guide for the Care and Use of Laboratory Animals available. American Physiological Society. Physiologist. 1996; 39(4):199, 208-11. View

12.

Beauchesne P . Fotemustine: a third-generation nitrosourea for the treatment of recurrent malignant gliomas. Cancers (Basel). 2013; 4(1):77-87. PMC: 3712672. DOI: 10.3390/cancers4010077. View

13.

Arensman M, Kovochich A, Kulikauskas R, Lay A, Yang P, Li X . WNT7B mediates autocrine Wnt/β-catenin signaling and anchorage-independent growth in pancreatic adenocarcinoma. Oncogene. 2013; 33(7):899-908. PMC: 3923845. DOI: 10.1038/onc.2013.23. View

14.

Spizzo R, Almeida M, Colombatti A, Calin G . Long non-coding RNAs and cancer: a new frontier of translational research?. Oncogene. 2012; 31(43):4577-87. PMC: 3433647. DOI: 10.1038/onc.2011.621. View

15.

Naemura M, Kuroki M, Tsunoda T, Arikawa N, Sawata Y, Shirasawa S . The Long Noncoding RNA Is Involved in the Regulation of Cell Proliferation. Anticancer Res. 2017; 38(1):77-81. DOI: 10.21873/anticanres.12194. View

16.

Liu C, Tu Y, Sun X, Jiang J, Jin X, Bo X . Wnt/beta-Catenin pathway in human glioma: expression pattern and clinical/prognostic correlations. Clin Exp Med. 2010; 11(2):105-12. DOI: 10.1007/s10238-010-0110-9. View

17.

Jansen M, Yip S, Louis D . Molecular pathology in adult gliomas: diagnostic, prognostic, and predictive markers. Lancet Neurol. 2010; 9(7):717-26. PMC: 3079431. DOI: 10.1016/S1474-4422(10)70105-8. View

18.

Baryawno N, Sveinbjornsson B, Eksborg S, Chen C, Kogner P, Johnsen J . Small-molecule inhibitors of phosphatidylinositol 3-kinase/Akt signaling inhibit Wnt/beta-catenin pathway cross-talk and suppress medulloblastoma growth. Cancer Res. 2009; 70(1):266-76. DOI: 10.1158/0008-5472.CAN-09-0578. View

19.

Baarsma H, Konigshoff M, Gosens R . The WNT signaling pathway from ligand secretion to gene transcription: molecular mechanisms and pharmacological targets. Pharmacol Ther. 2013; 138(1):66-83. DOI: 10.1016/j.pharmthera.2013.01.002. View

20.

Chen L, Zhang J, Feng Y, Li R, Sun X, Du W . MiR-410 regulates MET to influence the proliferation and invasion of glioma. Int J Biochem Cell Biol. 2012; 44(11):1711-7. DOI: 10.1016/j.biocel.2012.06.027. View