Antigen-specific CD8 T Cells in Cell Cycle Circulate in the Blood After Vaccination

Overview

Journal Scand J Immunol

Specialty Allergy & Immunology

Date 2018 Nov 30

PMID 30488973

Citations 13

Authors

Sonia Simonetti

Ambra Natalini

Antonella Folgori

Stefania Capone

Alfredo Nicosia

Angela Santoni

Francesca Di Rosa

Affiliations

Soon will be listed here.

Abstract

Although clonal expansion is a hallmark of adaptive immunity, the location(s) where antigen-responding T cells enter cell cycle and complete it have been poorly explored. This lack of knowledge stems partially from the limited experimental approaches available. By using Ki67 plus DNA staining and a novel strategy for flow cytometry analysis, we distinguished antigen-specific CD8 T cells in G , in G and in S-G /M phases of cell cycle after intramuscular vaccination of BALB/c mice with antigen-expressing viral vectors. Antigen-specific cells in S-G /M were present at early times after vaccination in lymph nodes (LNs), spleen and, surprisingly, also in the blood, which is an unexpected site for cycling of normal non-leukaemic cells. Most proliferating cells had high scatter profile and were undetected by current criteria of analysis, which under-estimated up to 6 times antigen-specific cell frequency in LNs. Our discovery of cycling antigen-specific CD8 T cells in the blood opens promising translational perspectives.

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