Immunological T-cell Memory in the in Vitro-induced Experimental Autoimmune Orchitis: Specificity of the Reaction and Tissue Distribution of the Autoantigens

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1978 Jan 1

PMID 304879

Citations 6

Authors

H Wekerle

Affiliations

Soon will be listed here.

Abstract

Immunological memory has been induced in vitro against testicular autoantigens by priming normal rat T lymphocytes against autologous testis cells, and by permitting the isolated blast cells to revert back to small secondary lymphocytes (secondary EAO cells) in the absence of the priming antigen. The secondary EAO cells vigorously respond in a secondary response when reconfronted with syngeneic testis or lymphoid cells. Their responsiveness to nonself stimulator cells is, however, reduced. Secondary cells derived from concanavalin A-stimulated blasts, do not show that pattern of specificity. The specificity of the secondary EAO cells is definite, and cannot be affected by further culture on allogeneic fibroblasts, which are antigenic for unprimed T lymphocytes. At least part of the autoantigens are determined by the major histocompatibility gene complex (MHC). Factors provided by the culture system do not appear to determine the specificity of this reaction. Only minor cell populations can restimulate secondary EAO cells. One of these populations is presumably phage-like cells within the lymphoid populations can elicit a secondary EAO response. Thus, the autoantigens relevant in the secondary EAO response are either MHC antigens restricted to these testicular and lymphoid subpopulations, or MHC antigens recognized in conjunction with organ-specific non-MHC determinants.

Citing Articles

Delayed hypersensitivity to syngeneic or xenogeneic testicular cells in guinea-pigs.

Yoshida S, Nomoto K, Himeno K, Sanui H Immunology. 1981; 42(3):445-9.

PMID: 7203531 PMC: 1458443.

Sex difference in delayed footpad reaction to syngeneic testicular cells in C3H/He mice.

Yoshida S, Ohhara R, Sanui H, Nomoto K Immunology. 1983; 48(3):537-42.

PMID: 6826204 PMC: 1454030.

Induction of tolerance in vitro by autologous murine testicular cells.

Hurtenbach U, Morgenstern F, Bennett D J Exp Med. 1980; 151(4):827-38.

PMID: 6445394 PMC: 2185828. DOI: 10.1084/jem.151.4.827.

Experimental allergic orchitis in mice. I. Genetic control of susceptibility and resistance to induction of autoimmune orchitis.

Teuscher C, Smith S, Goldberg E, Shearer G, Tung K Immunogenetics. 1985; 22(4):323-33.

PMID: 3932196 DOI: 10.1007/BF00430916.

Immune response to syngeneic or autologous testicular cells in mice. I. Augmented delayed footpad reaction in cyclophosphamide-treated mice.

Yoshida S, Nomoto K, Himeno K, Takeya K Clin Exp Immunol. 1979; 38(2):211-7.

PMID: 527259 PMC: 1537856.

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