CHESS: a New Human Gene Catalog Curated from Thousands of Large-scale RNA Sequencing Experiments Reveals Extensive Transcriptional Noise

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2018 Nov 30

PMID 30486838

Citations 159

Authors

Mihaela Pertea

Alaina Shumate

Geo Pertea

Ales Varabyou

Florian P Breitwieser

Yu-Chi Chang

Anil K Madugundu

Akhilesh Pandey

Steven L Salzberg

Affiliations

Soon will be listed here.

Abstract

We assembled the sequences from deep RNA sequencing experiments by the Genotype-Tissue Expression (GTEx) project, to create a new catalog of human genes and transcripts, called CHESS. The new database contains 42,611 genes, of which 20,352 are potentially protein-coding and 22,259 are noncoding, and a total of 323,258 transcripts. These include 224 novel protein-coding genes and 116,156 novel transcripts. We detected over 30 million additional transcripts at more than 650,000 genomic loci, nearly all of which are likely nonfunctional, revealing a heretofore unappreciated amount of transcriptional noise in human cells. The CHESS database is available at http://ccb.jhu.edu/chess .

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