Antioxidant and Anti-inflammatory Functionality of Ten Sri Lankan Seaweed Extracts Obtained by Carbohydrase Assisted Extraction

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2018 Nov 29

PMID 30483441

Citations 15

Authors

I P Shanura Fernando

K K Asanka Sanjeewa

Kalpa W Samarakoon

Won Woo Lee

Hyun-Soo Kim

P Ranasinghe

U K D S S Gunasekara

You-Jin Jeon

Affiliations

Soon will be listed here.

Abstract

Enzyme-assisted extraction is a cost-effective, safe, and efficient method to obtain bioactives from plant materials. During this study, 10 different marine algae from Sri Lanka were individually extracted by using five commercial food-grade carbohydrases. The enzymatic and water extracts of the seaweeds were analyzed for their antioxidant and anti-inflammatory activities. The highest DPPH, hydrogen peroxide (HO) and intracellular HO scavenging abilities were observed from the Celluclast extract of (CSp). CSp exerted protective effects against oxidative stress-induced cell death in hydrogen peroxide-induced Chang cells and in model zebrafish. The Celluclast extract of (CCm) showed the strongest anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages (IC = 44.47 µg/mL) and in model zebrafish. CCm inhibited the levels of iNOS, COX-2, PGE, and TNF-α in LPS stimulated RAW 264.7 macrophages. Hence, CSp and CCm could be utilized in developing functional ingredients for foods, and cosmeceuticals.

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References

Sanjeewa K, Fernando I, Kim E, Ahn G, Jee Y, Jeon Y . Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed in RAW 264.7 cells. Nutr Res Pract. 2017; 11(1):3-10. PMC: 5300944. DOI: 10.4162/nrp.2017.11.1.3. View

Fernando I, Nah J, Jeon Y . Potential anti-inflammatory natural products from marine algae. Environ Toxicol Pharmacol. 2016; 48:22-30. DOI: 10.1016/j.etap.2016.09.023. View

Jiao G, Yu G, Zhang J, Ewart H . Chemical structures and bioactivities of sulfated polysaccharides from marine algae. Mar Drugs. 2011; 9(2):196-223. PMC: 3093253. DOI: 10.3390/md9020196. View

Coussens L, Werb Z . Inflammation and cancer. Nature. 2002; 420(6917):860-7. PMC: 2803035. DOI: 10.1038/nature01322. View

Ko S, Jeon Y . Anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue in lipopolysaccharide-stimulated RAW 264.7 macrophages and in vivo zebrafish model. Nutr Res Pract. 2015; 9(3):219-26. PMC: 4460052. DOI: 10.4162/nrp.2015.9.3.219. View

Fernando I, Sanjeewa K, Samarakoon K, Lee W, Kim H, Kang N . A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses. Int J Biol Macromol. 2017; 104(Pt A):1185-1193. DOI: 10.1016/j.ijbiomac.2017.07.031. View

Xu H, Barnes G, Yang Q, Tan G, Yang D, Chou C . Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest. 2003; 112(12):1821-30. PMC: 296998. DOI: 10.1172/JCI19451. View

Hehemann J, Correc G, Barbeyron T, Helbert W, Czjzek M, Michel G . Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota. Nature. 2010; 464(7290):908-12. DOI: 10.1038/nature08937. View

Chandler S, Dodds J . The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of solanum laciniatum. Plant Cell Rep. 2013; 2(4):205-8. DOI: 10.1007/BF00270105. View

10.

Hansson G . Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med. 2005; 352(16):1685-95. DOI: 10.1056/NEJMra043430. View

11.

Pham-Huy L, He H, Pham-Huy C . Free radicals, antioxidants in disease and health. Int J Biomed Sci. 2013; 4(2):89-96. PMC: 3614697. View

12.

Rastogi R, Singh S, Hader D, Sinha R . Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem Biophys Res Commun. 2010; 397(3):603-7. DOI: 10.1016/j.bbrc.2010.06.006. View

13.

Rose M, Lewis J, Langford N, Baxter M, Origgi S, Barber M . Arsenic in seaweed--forms, concentration and dietary exposure. Food Chem Toxicol. 2007; 45(7):1263-7. DOI: 10.1016/j.fct.2007.01.007. View

14.

Wijesinghe W, Jeon Y . Enzyme-assistant extraction (EAE) of bioactive components: a useful approach for recovery of industrially important metabolites from seaweeds: a review. Fitoterapia. 2011; 83(1):6-12. DOI: 10.1016/j.fitote.2011.10.016. View

15.

Fernando I, Kim M, Son K, Jeong Y, Jeon Y . Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach. J Med Food. 2016; 19(7):615-28. DOI: 10.1089/jmf.2016.3706. View

16.

Premakumara G, Ratnasooriya W, Tillekeratne L . Isolation of a non-steroidal contragestative agent from Sri Lankan marine red alga, Gelidiella acerosa. Contraception. 1996; 54(6):379-83. DOI: 10.1016/s0010-7824(96)00198-9. View

17.

Kang M, Cha S, Wijesinghe W, Kang S, Lee S, Kim E . Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo. Food Chem. 2013; 138(2-3):950-5. DOI: 10.1016/j.foodchem.2012.11.005. View

18.

Li J, Vederas J . Drug discovery and natural products: end of an era or an endless frontier?. Science. 2009; 325(5937):161-5. DOI: 10.1126/science.1168243. View