Effect of Intra-ovarian Injection of Mesenchymal Stem Cells in Aged Mares

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2018 Nov 25

PMID 30470961

Citations 16

Authors

Sicilia T Grady

Ashlee E Watts

James A Thompson

M Cecilia T Penedo

Kranti Konganti

Katrin Hinrichs

Affiliations

Soon will be listed here.

Abstract

Purpose: This study aims to determine if intra-ovarian injection of bone marrow-derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females.

Methods: Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing.

Results: Injection of MSCs was not associated with significant changes in follicle number, oocyte recovery rate on follicle aspiration, oocyte maturation rate, or blastocyst rate after ICSI in aged mares, or in changes in follicle number in young mares. There were no significant changes in peripheral AMH concentrations, indicating a lack of effect on growing follicles. MSC donor DNA was not recovered in granulosa cells or in ovarian tissue, indicating lack of persistence of injected MSC. RNA sequencing revealed significant differences in gene expression between MSC- and vehicle-injected ovaries.

Conclusions: Intra-ovarian injection of bone marrow-derived MSCs altered gene expression but did not improve ovarian function in aged mares.

Citing Articles

Stem cell-based therapeutic potential in female ovarian aging and infertility.

Cui X, Jing X J Ovarian Res. 2024; 17(1):171.

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Current Status and Future Prospects of Stem Cell Therapy for Infertile Patients with Premature Ovarian Insufficiency.

Kim H, Kim T Biomolecules. 2024; 14(2).

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Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses.

Ren W, Wang J, Zeng Y, Wang T, Meng J, Yao X Front Endocrinol (Lausanne). 2024; 15:1346260.

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Use of mesenchymal stem cells to enhance or restore fertility potential: a systematic review of available experimental strategies.

Cacciottola L, Vitale F, Donnez J, Dolmans M Hum Reprod Open. 2023; 2023(4):hoad040.

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Feline Wharton's jelly-derived mesenchymal stem cells as a feeder layer for oocytes maturation and embryos culture .

Baouche M, Ochota M, Mermillod P, Locatelli Y, Nizanski W Front Vet Sci. 2023; 10:1252484.

PMID: 37869498 PMC: 10590214. DOI: 10.3389/fvets.2023.1252484.

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