Calcium Transfer Across the Outer Mantle Epithelium in the Pacific Oyster,

Overview

Journal Proc Biol Sci

Specialty Biology

Date 2018 Nov 16

PMID 30429301

Citations 13

Authors

J Kirsikka Sillanpaa

Henrik Sundh

Kristina S Sundell

Affiliations

Soon will be listed here.

Abstract

Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca transfer performed by the OME of the Pacific oyster, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis-Menten kinetics and is characterized by a of 6.2 mM and a of 3.3 nM min The transcellular transfer of Ca accounts for approximately 60% of the total Ca transfer across the OME of s at environmental Ca concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca-channels, plasma-membrane Ca-ATPase and Na/Ca-exchanger all participate in the transcellular Ca transfer across the OME and a model for this Ca transfer is presented and discussed.

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References

Oliveira P, Lopes I, Barrias C, Rebelo da Costa A . H+-ATPase of crude homogenate of the outer mantle epithelium of Anodonta cygnea. Comp Biochem Physiol A Mol Integr Physiol. 2004; 139(4):425-32. DOI: 10.1016/j.cbpb.2004.07.004. View

Mount A, Wheeler A, Paradkar R, Snider D . Hemocyte-mediated shell mineralization in the eastern oyster. Science. 2004; 304(5668):297-300. DOI: 10.1126/science.1090506. View

Bautista D, Hoth M, Lewis R . Enhancement of calcium signalling dynamics and stability by delayed modulation of the plasma-membrane calcium-ATPase in human T cells. J Physiol. 2002; 541(Pt 3):877-94. PMC: 2290354. DOI: 10.1113/jphysiol.2001.016154. View

Strehler E, Zacharias D . Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps. Physiol Rev. 2001; 81(1):21-50. DOI: 10.1152/physrev.2001.81.1.21. View

Strehler E, Treiman M . Calcium pumps of plasma membrane and cell interior. Curr Mol Med. 2004; 4(3):323-35. DOI: 10.2174/1566524043360735. View

Chaudhary J, Walia M, Matharu J, Escher E, Grover A . Caloxin: a novel plasma membrane Ca2+ pump inhibitor. Am J Physiol Cell Physiol. 2001; 280(4):C1027-30. DOI: 10.1152/ajpcell.2001.280.4.C1027. View

Joubert C, Piquemal D, Marie B, Manchon L, Pierrat F, Zanella-Cleon I . Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization. BMC Genomics. 2010; 11:613. PMC: 3091754. DOI: 10.1186/1471-2164-11-613. View

Shi Y, Yu C, Gu Z, Zhan X, Wang Y, Wang A . Characterization of the pearl oyster (Pinctada martensii) mantle transcriptome unravels biomineralization genes. Mar Biotechnol (NY). 2012; 15(2):175-87. DOI: 10.1007/s10126-012-9476-x. View

Smith C . Cellular and chemical events during enamel maturation. Crit Rev Oral Biol Med. 1998; 9(2):128-61. DOI: 10.1177/10454411980090020101. View

10.

Jost N, Nagy N, Corici C, Kohajda Z, Horvath A, Acsai K . ORM-10103, a novel specific inhibitor of the Na+/Ca2+ exchanger, decreases early and delayed afterdepolarizations in the canine heart. Br J Pharmacol. 2013; 170(4):768-78. PMC: 3799592. DOI: 10.1111/bph.12228. View

11.

Pande J, Szewczyk M, Kuszczak I, Grover S, Escher E, Grover A . Functional effects of caloxin 1c2, a novel engineered selective inhibitor of plasma membrane Ca(2+)-pump isoform 4, on coronary artery. J Cell Mol Med. 2008; 12(3):1049-60. PMC: 4401146. DOI: 10.1111/j.1582-4934.2008.00140.x. View

12.

Xue Q, Gauthier J, Schey K, Li Y, Cooper R, Anderson R . Identification of a novel metal binding protein, segon, in plasma of the eastern oyster, Crassostrea virginica. Comp Biochem Physiol B Biochem Mol Biol. 2012; 163(1):74-85. DOI: 10.1016/j.cbpb.2012.05.002. View

13.

Sillanpaa J, Ramesh K, Melzner F, Sundh H, Sundell K . Calcium mobilisation following shell damage in the Pacific oyster, Crassostrea gigas. Mar Genomics. 2016; 27:75-83. DOI: 10.1016/j.margen.2016.03.001. View

14.

Sillanpaa J, Sundh H, Sundell K . Calcium transfer across the outer mantle epithelium in the Pacific oyster, . Proc Biol Sci. 2018; 285(1891). PMC: 6253367. DOI: 10.1098/rspb.2018.1676. View

15.

Fromter E, Diamond J . Route of passive ion permeation in epithelia. Nat New Biol. 1972; 235(53):9-13. DOI: 10.1038/newbio235009a0. View

16.

Pott C, Henderson S, Goldhaber J, Philipson K . Na+/Ca2+ exchanger knockout mice: plasticity of cardiac excitation-contraction coupling. Ann N Y Acad Sci. 2007; 1099:270-5. DOI: 10.1196/annals.1387.015. View

17.

Zhang G, Fang X, Guo X, Li L, Luo R, Xu F . The oyster genome reveals stress adaptation and complexity of shell formation. Nature. 2012; 490(7418):49-54. DOI: 10.1038/nature11413. View

18.

Xiang L, Kong W, Su J, Su J, Liang J, Zhang G . Amorphous calcium carbonate precipitation by cellular biomineralization in mantle cell cultures of Pinctada fucata. PLoS One. 2014; 9(11):e113150. PMC: 4236139. DOI: 10.1371/journal.pone.0113150. View

19.

Nair P, Robinson W . Calcium speciation and exchange between blood and extrapallial fluid of the quahog Mercenaria mercenaria (L.). Biol Bull. 1998; 195(1):43-51. DOI: 10.2307/1542774. View

20.

Domi T, Di Leva F, Fedrizzi L, Rimessi A, Brini M . Functional specificity of PMCA isoforms?. Ann N Y Acad Sci. 2007; 1099:237-46. DOI: 10.1196/annals.1387.043. View