The Role of IS-Borne P Promoters in the Expression of Antibiotic Resistance Genes

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Nov 15

PMID 30425694

Citations 7

Authors

Claire Lallement

Cecile Pasternak

Marie-Cecile Ploy

Thomas Jove

Affiliations

Soon will be listed here.

Abstract

The IS (Insertion sequence Common Region) element is the most widespread member of the IS family, and is frequently present within γ-proteobacteria that occur in clinical settings. IS is always associated with the 3'Conserved Segment (3'CS) of class 1 integrons. IS contains outward-oriented promoters P, that may contribute to the expression of downstream genes. In IS, there are two P promoters named P and P. We performed an analysis of all publically available IS sequences and identified numerous downstream genes that mainly encode antibiotic resistance genes and that are oriented in the same direction as the P promoters. Here, we showed that both P and P significantly increase the expression of the downstream genes and . Our data highlight the role of IS in the expression of antibiotic resistance genes, which may explain why IS is so frequent in clinical settings.

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