A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2018 Nov 15

PMID 30424121

Citations 5

Authors

Roberto Fabian Jr

Christopher Tyson

Pamela L Tuma

Ian Pegg

Abhijit Sarkar

Affiliations

Soon will be listed here.

Abstract

We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force⁻extension data from our experiments show high-resolution detection of changes in the DNA tether's extension: ~0.5 pN in the force and <10 nm change in extension. We calibrate our instrument using multiple orthogonal techniques including the well-characterized DNA overstretching transition. We also quantify the repeatability of force and extension measurements, and present data on the behavior of the overstretching transition under varying salt conditions. The design and experimental protocols are described in detail, which should enable straightforward reproduction of the tweezers.

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A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins.

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