Role of a Serine Protease Gene (AccSp1) from Apis Cerana Cerana in Abiotic Stress Responses and Innate Immunity

Overview

Journal Cell Stress Chaperones

Publisher Elsevier

Specialty Cell Biology

Date 2018 Nov 11

PMID 30413995

Citations 3

Authors

Lijun Gao

Lijun Wang

Xinxin Yang

Ying Wang

Zhenguo Liu

Baohua Xu

Xingqi Guo

Affiliations

Soon will be listed here.

Abstract

Clip-domain serine proteases (Clip-SPs) mediate innate immunity and embryonic development in insects. However, the function of Clip-SPs in Apis cerana cerana is little known. Here, a Clip-SP gene, AccSp1, was identified. AccSp1 was mainly detected in third and sixth day instar larvae, dark-eyed pupae, and adults (1and 30 days post-emergence). In addition, AccSp1 was expressed at its highest level in the venom gland and epidermis than tentacle, abdomen, muscle, honey sac, head, leg, chest, hemolymph, rectum, and midgut. AccSp1 was induced by 4, 24, and 44 °C; HO; CdCl HgCl; and pesticides (paraquat, pyridaben, and methomyl) and was inhibited by UV light and cyhalothrin treatments. When adults that had been pretreated with dsRNA 6 h prior (knocking AccSp1 down) were challenged with Bacillus bombysepticus for 18 h, the survival rate of bees greatly decreased, the activity of PO (phenoloxidase) was reduced, revealing that AccSp1 may play a critical role in assisting bees to survive the microbial infection and participate in regulating PO activity. The antioxidant enzymatic activities of catalase, peroxidase, and superoxide dismutase; the contents of hydrogen peroxide and malondialdehyde; and the ratio of NADP/NADPH were all lower in samples containing dsRNA-AccSp1 interference than in control groups, but the content of carbonyl was not significantly different. These findings suggest the knockdown of AccSp1 may influence melanization so that the antioxidant enzyme activities and the harmful metabolites decreased. These results collectively suggest that AccSp1 plays critical roles in abiotic stresses responses and resistance to pathogens.

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References

An M, Choi C . Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: effects on hemolymph and biochemical parameters. Comp Biochem Physiol B Biochem Mol Biol. 2009; 155(1):34-42. DOI: 10.1016/j.cbpb.2009.09.008. View

Li J, Qin H, Wu J, Sadd B, Wang X, Evans J . The prevalence of parasites and pathogens in Asian honeybees Apis cerana in China. PLoS One. 2012; 7(11):e47955. PMC: 3492380. DOI: 10.1371/journal.pone.0047955. View

Yu X, Zhu Y, Ma C, Fabrick J, Kanost M . Pattern recognition proteins in Manduca sexta plasma. Insect Biochem Mol Biol. 2002; 32(10):1287-93. DOI: 10.1016/s0965-1748(02)00091-7. View

An C, Ishibashi J, Ragan E, Jiang H, Kanost M . Functions of Manduca sexta hemolymph proteinases HP6 and HP8 in two innate immune pathways. J Biol Chem. 2009; 284(29):19716-26. PMC: 2740596. DOI: 10.1074/jbc.M109.007112. View

Feng M, Ramadan H, Han B, Fang Y, Li J . Hemolymph proteome changes during worker brood development match the biological divergences between western honey bees (Apis mellifera) and eastern honey bees (Apis cerana). BMC Genomics. 2014; 15:563. PMC: 4111844. DOI: 10.1186/1471-2164-15-563. View

Kim M, Baek M, Lee M, Park J, Lee S, Soderhall K . A new easter-type serine protease cleaves a masquerade-like protein during prophenoloxidase activation in Holotrichia diomphalia larvae. J Biol Chem. 2002; 277(42):39999-40004. DOI: 10.1074/jbc.M205508200. View

Casewell N, Wuster W, Vonk F, Harrison R, Fry B . Complex cocktails: the evolutionary novelty of venoms. Trends Ecol Evol. 2012; 28(4):219-29. DOI: 10.1016/j.tree.2012.10.020. View

Mavrouli M, Tsakas S, Theodorou G, Lampropoulou M, Marmaras V . MAP kinases mediate phagocytosis and melanization via prophenoloxidase activation in medfly hemocytes. Biochim Biophys Acta. 2005; 1744(2):145-56. DOI: 10.1016/j.bbamcr.2005.04.011. View

Corona M, Robinson G . Genes of the antioxidant system of the honey bee: annotation and phylogeny. Insect Mol Biol. 2006; 15(5):687-701. PMC: 1847502. DOI: 10.1111/j.1365-2583.2006.00695.x. View

10.

Lee K, Kim S, Park N, Kim I, Kang P, Sohn B . Characterization of a silkworm thioredoxin peroxidase that is induced by external temperature stimulus and viral infection. Insect Biochem Mol Biol. 2004; 35(1):73-84. DOI: 10.1016/j.ibmb.2004.09.008. View

11.

Jiang H, Wang Y, Gu Y, Guo X, Zou Z, Scholz F . Molecular identification of a bevy of serine proteinases in Manduca sexta hemolymph. Insect Biochem Mol Biol. 2005; 35(8):931-43. PMC: 2042105. DOI: 10.1016/j.ibmb.2005.03.009. View

12.

Gorman M, Wang Y, Jiang H, Kanost M . Manduca sexta hemolymph proteinase 21 activates prophenoloxidase-activating proteinase 3 in an insect innate immune response proteinase cascade. J Biol Chem. 2007; 282(16):11742-9. PMC: 2042106. DOI: 10.1074/jbc.M611243200. View

13.

Nappi A, Christensen B . Melanogenesis and associated cytotoxic reactions: applications to insect innate immunity. Insect Biochem Mol Biol. 2005; 35(5):443-59. DOI: 10.1016/j.ibmb.2005.01.014. View

14.

Fry B, Roelants K, Champagne D, Scheib H, Tyndall J, King G . The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms. Annu Rev Genomics Hum Genet. 2009; 10:483-511. DOI: 10.1146/annurev.genom.9.081307.164356. View

15.

Boman H . Peptide antibiotics and their role in innate immunity. Annu Rev Immunol. 1995; 13:61-92. DOI: 10.1146/annurev.iy.13.040195.000425. View

16.

Gao L, Wang H, Liu Z, Liu S, Zhao G, Xu B . The initial analysis of a serine proteinase gene (AccSp10) from Apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses. Cell Stress Chaperones. 2017; 22(6):867-877. PMC: 5655375. DOI: 10.1007/s12192-017-0818-5. View

17.

Dolling R, Becker D, Hawat S, Koch M, Schwarzenberger A, Zeis B . Adjustments of serine proteases of Daphnia pulex in response to temperature changes. Comp Biochem Physiol B Biochem Mol Biol. 2016; 194-195:1-10. DOI: 10.1016/j.cbpb.2016.01.001. View

18.

Dziarski R . Peptidoglycan recognition proteins (PGRPs). Mol Immunol. 2003; 40(12):877-86. DOI: 10.1016/j.molimm.2003.10.011. View

19.

Hasegawa T . Tyrosinase-expressing neuronal cell line as in vitro model of Parkinson's disease. Int J Mol Sci. 2010; 11(3):1082-9. PMC: 2869230. DOI: 10.3390/ijms11031082. View

20.

Jiang H, Kanost M . The clip-domain family of serine proteinases in arthropods. Insect Biochem Mol Biol. 2000; 30(2):95-105. DOI: 10.1016/s0965-1748(99)00113-7. View