Rapid Capture and Analysis of Airborne Staphylococcus Aureus in the Hospital Using a Microfluidic Chip

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2018 Nov 9

PMID 30404341

Citations 8

Authors

Xiran Jiang

Yingchao Liu

Qi Liu

Wenwen Jing

Kairong Qin

Guodong Sui

Affiliations

Soon will be listed here.

Abstract

In this study we developed a microfluidic chip for the rapid capture, enrichment and detection of airborne () . The whole analysis took about 4 h and 40 min from airborne sample collection to loop-mediated isothermal amplification (LAMP), with a detection limit down to about 27 cells. The process did not require DNA purification. The chip was validated using standard bacteria bioaerosol and was directly used for clinical airborne pathogen sampling in hospital settings. This is the first report on the capture and analysis of airborne using a novel microfluidic technique, a process that could have a very promising platform for hospital airborne infection prevention (HAIP).

Citing Articles

Rapid In-Field Detection of Airborne Pathogens Using Loop-Mediated Isothermal Amplification (LAMP).

Bani A, Whitby C, Colbeck I, Dumbrell A, Ferguson R Microorganisms. 2025; 12(12.

PMID: 39770780 PMC: 11678261. DOI: 10.3390/microorganisms12122578.

Development of a Novel Electrostatic-Based Bioaerosol Sampler.

Pang Z, Shi L, Liu W, Liu W, Tian X, Wang M Micromachines (Basel). 2024; 15(9).

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A Molecular Approach for Detecting Bacteria and Fungi in Healthcare Environment Aerosols: A Systematic Review.

Matys J, Kensy J, Gedrange T, Zawislak I, Grzech-Lesniak K, Dobrzynski M Int J Mol Sci. 2024; 25(8).

PMID: 38673740 PMC: 11050369. DOI: 10.3390/ijms25084154.

On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies.

Rastmanesh A, Boruah J, Lee M, Park S Biosensors (Basel). 2024; 14(3).

PMID: 38534229 PMC: 10968652. DOI: 10.3390/bios14030122.

Lab-on-a-Chip Platforms for Airborne Particulate Matter Applications: A Review of Current Perspectives.

Ezrre S, Reyna M, Anguiano C, Avitia R, Marquez H Biosensors (Basel). 2022; 12(4).

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