» Articles » PMID: 6289329

Transposon Tn10 Provides a Promoter for Transcription of Adjacent Sequences

Overview
Specialty Science
Date 1982 Aug 1
PMID 6289329
Citations 80
Authors
Affiliations
Soon will be listed here.
Abstract

Promoters located within the Tn10 insertion element cause transcription of "host" sequences adjacent to both ends of the inserted Tn10 element. These promoters are usually not observed in genetic experiments because their transcripts are efficiently terminated at nearby rho-dependent termination sites. The observations presented here provide an explanation for several confusing aspects of transposon behavior and suggest the possibility that many transposons possess promoters that have escaped detection for similar reasons.

Citing Articles

A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli.

Tan J, Hu Q, Scrutton N Microb Cell Fact. 2024; 23(1):279.

PMID: 39415159 PMC: 11481808. DOI: 10.1186/s12934-024-02548-1.


Differential Afa/Dr Fimbriae Expression in the Multidrug-Resistant Escherichia coli ST131 Clone.

Alvarez-Fraga L, Phan M, Goh K, Nhu N, Hancock S, Allsopp L mBio. 2022; 13(1):e0351921.

PMID: 35038925 PMC: 8764528. DOI: 10.1128/mbio.03519-21.


Upregulation of a homolog by transposon insertion promotes leafy head development in lettuce.

Yu C, Yan C, Liu Y, Liu Y, Jia Y, Lavelle D Proc Natl Acad Sci U S A. 2020; 117(52):33668-33678.

PMID: 33288708 PMC: 7776633. DOI: 10.1073/pnas.2019698117.


The N-terminus of IFT46 mediates intraflagellar transport of outer arm dynein and its cargo-adaptor ODA16.

Hou Y, Witman G Mol Biol Cell. 2017; 28(18):2420-2433.

PMID: 28701346 PMC: 5576905. DOI: 10.1091/mbc.E17-03-0172.


A Natural Vibrio parahaemolyticus Δ Mutant Kills Shrimp but Produces neither Pir Toxins nor Acute Hepatopancreatic Necrosis Disease Lesions.

Phiwsaiya K, Charoensapsri W, Taengphu S, Dong H, Sangsuriya P, Nguyen G Appl Environ Microbiol. 2017; 83(16).

PMID: 28576761 PMC: 5541212. DOI: 10.1128/AEM.00680-17.


References
1.
Schmidt F, Jorgensen R, de Wilde M, Davies J . A specific tetracycline-induced, low-molecular-weight RNA encoded by the inverted repeat of Tn10 (IS10). Plasmid. 1981; 6(1):148-50. DOI: 10.1016/0147-619x(81)90061-5. View

2.
Reynolds A, Felton J, Wright A . Insertion of DNA activates the cryptic bgl operon in E. coli K12. Nature. 1981; 293(5834):625-9. DOI: 10.1038/293625a0. View

3.
Brewster J, Morgan E . Tn9 and IS1 inserts in a ribosomal ribonucleic acid operon of Escherichia coli are incompletely polar. J Bacteriol. 1981; 148(3):897-903. PMC: 216290. DOI: 10.1128/jb.148.3.897-903.1981. View

4.
Blazey D, BURNS R . Transcriptional activity of the transposable element Tn10 in the Salmonella typhimurium ilvGEDA operon. Proc Natl Acad Sci U S A. 1982; 79(16):5011-5. PMC: 346816. DOI: 10.1073/pnas.79.16.5011. View

5.
McCLINTOCK B . Controlling elements and the gene. Cold Spring Harb Symp Quant Biol. 1956; 21:197-216. DOI: 10.1101/sqb.1956.021.01.017. View