A FRET Biosensor for Necroptosis Uncovers Two Different Modes of the Release of DAMPs

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Oct 28

PMID 30367066

Citations 39

Authors

Shin Murai

Yoshifumi Yamaguchi

Yoshitaka Shirasaki

Mai Yamagishi

Ryodai Shindo

Joanne M Hildebrand

Ryosuke Miura

Osamu Nakabayashi

Mamoru Totsuka

Taichiro Tomida

Satomi Adachi-Akahane

Sotaro Uemura

John Silke

Hideo Yagita

Masayuki Miura

Hiroyasu Nakano

Affiliations

Soon will be listed here.

Abstract

Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like (MLKL). While danger-associated molecular pattern (DAMP)s are involved in various pathological conditions and released from dead cells, the underlying mechanisms are not fully understood. Here we develop a fluorescence resonance energy transfer (FRET) biosensor, termed SMART (a sensor for MLKL activation by RIPK3 based on FRET). SMART is composed of a fragment of MLKL and monitors necroptosis, but not apoptosis or necrosis. Mechanistically, SMART monitors plasma membrane translocation of oligomerized MLKL, which is induced by RIPK3 or mutational activation. SMART in combination with imaging of the release of nuclear DAMPs and Live-Cell Imaging for Secretion activity (LCI-S) reveals two different modes of the release of High Mobility Group Box 1 from necroptotic cells. Thus, SMART and LCI-S uncover novel regulation of the release of DAMPs during necroptosis.

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