An Improved Method for Detecting Y Chromosomal DNA

Overview

Journal Hum Genet

Specialty Genetics

Date 1987 Jul 1

PMID 3036688

Citations 5

Authors

J R Stalvey

R P Erickson

Affiliations

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Abstract

The DNA probe Y97 was derived from a repeat sequence in the human Y centromere, a region which must be present in a mitotically functional Y chromosome. We have demonstrated that Y97, which detects a Y-specific 5.5-kb EcoRI fragment by Southern analysis, is very useful for the molecular detection of small amounts of Y-derived material and represents a significant improvement over previous tests for molecular diagnosis of sex. The male-female difference in hybridization was unequivocal even when only 25 ng of total DNA was used per lane. Furthermore, in mixing experiments the 5.5-kb EcoRI fragment was detectable even when only 5% of the total DNA was male. By increasing hybridization stringency, we have developed a rapid, sensitive, and accurate method to detect Y chromosomal DNA in unrestricted samples.

Citing Articles

An improved, non-isotopic method of screening cells from patients with abnormalities of sexual differentiation for Y chromosomal DNA content.

Witt M, Michalczak K, Latos-Bielenska A, Jaruzelska J, Kuczora I, Lopez M J Med Genet. 1993; 30(4):304-7.

PMID: 8487276 PMC: 1016338. DOI: 10.1136/jmg.30.4.304.

Cytogenetic and molecular analysis of sex-chromosome monosomy.

Hassold T, Benham F, Leppert M Am J Hum Genet. 1988; 42(4):534-41.

PMID: 2894760 PMC: 1715233.

A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction.

Witt M, Erickson R Hum Genet. 1989; 82(3):271-4.

PMID: 2567276 DOI: 10.1007/BF00291168.

Identification and characterization of normal length nonfluorescent Y chromosomes: cytogenetic analysis, southern hybridization and non-isotopic in situ hybridization.

Speleman F, Van der Auwera B, Mangelschots K, Vercruyssen M, Raap T, Wiegant J Hum Genet. 1990; 85(6):569-75.

PMID: 2227946 DOI: 10.1007/BF00193576.

Sex determination and species exclusion in forensic samples with probe cY97.

Fattorini P, Caccio S, Gustincich S, Wolfe J, Altamura B, Graziosi G Int J Legal Med. 1991; 104(5):247-50.

PMID: 1782143 DOI: 10.1007/BF01369578.

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