Standard Detection Protocol: PCR and RFLP Analyses Based on 16S RRNA Gene

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2018 Oct 27

PMID 30361997

Citations 5

Authors

Assunta Bertaccini

Samanta Paltrinieri

Nicoletta Contaldo

Affiliations

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Abstract

Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.

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