Protein-DNA Interactions in Vivo Upstream of a Cell Cycle-regulated Human H4 Histone Gene

Overview

Journal Science

Specialty Science

Date 1987 Jun 5

PMID 3035717

Citations 49

Authors

U Pauli

S Chrysogelos

G Stein

J Stein

H Nick

Affiliations

Soon will be listed here.

Abstract

Cell cycle-dependent histone genes are transcribed at a basal level throughout the cell cycle, with a three- to fivefold increase during early S phase. Protein-DNA interactions in the 5' promoter region of a cell cycle-regulated human H4 histone gene have been analyzed at single-nucleotide resolution in vivo. This region contains two sites, with four potential protein-binding domains, at which the DNA is protected from reaction with dimethyl sulfate in cells and from digestion with deoxyribonuclease I in nuclei. These protein-DNA interactions persist during all phases of the cell cycle and dissociate with 0.16 to 0.2M sodium chloride.

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