Quantification of Permethrin Resistance and Kdr Alleles in Florida Strains of Aedes Aegypti (L.) and Aedes Albopictus (Skuse)

Overview

Journal PLoS Negl Trop Dis

Specialties Infectious Diseases
Tropical Medicine

Date 2018 Oct 26

PMID 30356237

Citations 37

Authors

Alden S Estep

Neil D Sanscrainte

Christy M Waits

Sarah J Bernard

Aaron M Lloyd

Keira J Lucas

Eva A Buckner

Rajeev Vaidyanathan

Rachel Morreale

Lisa A Conti

James J Becnel

Affiliations

Soon will be listed here.

Abstract

Recent outbreaks of locally transmitted dengue and Zika viruses in Florida have placed more emphasis on integrated vector management plans for Aedes aegypti (L.) and Aedes albopictus Skuse. Adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. While pyrethroid resistance is common in Ae. aegypti worldwide and testing is recommended by CDC and WHO, resistance to this class of products has not been widely examined or quantified in Florida. To address this information gap, we performed the first study to quantify both pyrethroid resistance and genetic markers of pyrethroid resistance in Ae. aegypti and Ae. albopictus strains in Florida. Using direct topical application to measure intrinsic toxicity, we examined 21 Ae. aegypti strains from 9 counties and found permethrin resistance (resistance ratio (RR) = 6-61-fold) in all strains when compared to the susceptible ORL1952 control strain. Permethrin resistance in five strains of Ae. albopictus was very low (RR<1.6) even when collected from the same containers producing resistant Ae. aegypti. Characterization of two sodium channel kdr alleles associated with pyrethroid-resistance showed widespread distribution in 62 strains of Ae. aegypti. The 1534 phenylalanine to cysteine (F1534C) single nucleotide polymorphism SNP was fixed or nearly fixed in all strains regardless of RR. We observed much more variation in the 1016 valine to isoleucine (V1016I) allele and observed that an increasing frequency of the homozygous V1016I allele correlates strongly with increased RR (Pearson corr = 0.905). In agreement with previous studies, we observed a very low frequency of three kdr genotypes, IIFF, VIFF, and IIFC. In this study, we provide a statewide examination of pyrethroid resistance, and demonstrate that permethrin resistance and the genetic markers for resistance are widely present in FL Ae. aegypti. Resistance testing should be included in an effective management program.

Citing Articles

Detection of the 1016Gly and 989Pro Knockdown Resistance Mutations in Florida, USA .

Estep A, Sanscrainte N, Lamberg F, McStoots D, Gosselin S Insects. 2024; 15(11).

PMID: 39590462 PMC: 11594491. DOI: 10.3390/insects15110863.

Monitoring of pyrethroid resistance in Aedes aegypti: first report of double and triple kdr mutations in Buenos Aires Province.

Barrera-Illanes A, Ledesma L, Alvarez-Costa A, Balsalobre A, Toloza C, Hernandez-Maiztegui A Parasit Vectors. 2024; 17(1):458.

PMID: 39522041 PMC: 11549831. DOI: 10.1186/s13071-024-06547-4.

The L1014F Knockdown Resistance Mutation Is Not a Strong Correlate of Phenotypic Resistance to Pyrethroids in Florida Populations of .

Estep A, Sanscrainte N, Stuck J, Unlu I, Prasauskas A, Mundis S Insects. 2024; 15(3).

PMID: 38535392 PMC: 10971530. DOI: 10.3390/insects15030197.

Evaluation of a novel triple-action adulticide containing a pyrethroid, macrocyclic lactone, and fatty acid against pyrethroid-resistant Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae).

Lucas K, Heinig R, Lake L, Williams K, Parker-Crockett C, Bales R J Med Entomol. 2024; 61(3):701-709.

PMID: 38460150 PMC: 11078576. DOI: 10.1093/jme/tjae032.

Insecticide resistance of Miami-Dade Culex quinquefasciatus populations and initial field efficacy of a new resistance-breaking adulticide formulation.

Unlu I, Buckner E, Medina J, Vasquez C, Cabrera A, Romero-Weaver A PLoS One. 2024; 19(2):e0296046.

PMID: 38346028 PMC: 10861066. DOI: 10.1371/journal.pone.0296046.

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