The Glycosphingolipid MacCer Promotes Synaptic Bouton Formation in Drosophila by Interacting with Wnt

Overview

Journal Elife

Specialty Biology

Date 2018 Oct 26

PMID 30355446

Citations 9

Authors

Yan Huang

Sheng Huang

Coralie Di Scala

Qifu Wang

Hans H Wandall

Jacques Fantini

Yong Q Zhang

Affiliations

Soon will be listed here.

Abstract

Lipids are structural components of cellular membranes and signaling molecules that are widely involved in development and diseases, but the underlying molecular mechanisms are poorly understood, partly because of the vast variety of lipid species and complexity of synthetic and turnover pathways. From a genetic screen, we identify that mannosyl glucosylceramide (MacCer), a species of glycosphingolipid (GSL), promotes synaptic bouton formation at the neuromuscular junction (NMJ). Pharmacological and genetic analysis shows that the NMJ growth-promoting effect of MacCer depends on normal lipid rafts, which are known to be composed of sphingolipids, sterols and select proteins. MacCer positively regulates the synaptic level of Wnt1/Wingless (Wg) and facilitates presynaptic Wg signaling, whose activity is raft-dependent. Furthermore, a functional GSL-binding motif in Wg exhibiting a high affinity for MacCer is required for normal NMJ growth. These findings reveal a novel mechanism whereby the GSL MacCer promotes synaptic bouton formation via Wg signaling.

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