A Distinct Isoform of ZNF207 Controls Self-renewal and Pluripotency of Human Embryonic Stem Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Oct 24

PMID 30349051

Citations 19

Authors

Fang Fang

Ninuo Xia

Benjamin Angulo

Joseph Carey

Zackery Cady

Jens Durruthy-Durruthy

Theo Bennett

Vittorio Sebastiano

Renee A Reijo Pera

Affiliations

Soon will be listed here.

Abstract

Self-renewal and pluripotency in human embryonic stem cells (hESCs) depends upon the function of a remarkably small number of master transcription factors (TFs) that include OCT4, SOX2, and NANOG. Endogenous factors that regulate and maintain the expression of master TFs in hESCs remain largely unknown and/or uncharacterized. Here, we use a genome-wide, proteomics approach to identify proteins associated with the OCT4 enhancer. We identify known OCT4 regulators, plus a subset of potential regulators including a zinc finger protein, ZNF207, that plays diverse roles during development. In hESCs, ZNF207 partners with master pluripotency TFs to govern self-renewal and pluripotency while simultaneously controlling commitment of cells towards ectoderm through direct regulation of neuronal TFs, including OTX2. The distinct roles of ZNF207 during differentiation occur via isoform switching. Thus, a distinct isoform of ZNF207 functions in hESCs at the nexus that balances pluripotency and differentiation to ectoderm.

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