Spatial Variability of Microbial Communities in a Fractured Sedimentary Rock Matrix Impacted by a Mixed Organics Plume

Dissolved phase contaminants, transported by diffusion into the low permeability matrix of fractured sedimentary rock, pose a challenge to groundwater cleanup efforts because this stored mass may persist even when the upgradient source zone is removed. In this context, if contaminant biodegradation takes place within the low permeability matrix, plume persistence may be substantially reduced. Therefore, it is important to characterize microbial communities within the low permeability, rock matrix pores, instead of only from groundwater samples, which represent biomass from fast flowing fractures. This research relies on depth-discrete data from both core and groundwater samples collected from two locations representing a mid-plume and plume front condition within an aged, mixed organic contaminant plume in a sedimentary rock aquifer. Results from multiple analyte measurements on rock and groundwater indicate that biodegradation in the lower permeability matrix of fractured sedimentary rocks and the microbial consortia is spatially variable due to differences in hydrochemistry, redox conditions, and contaminant concentrations. Dechlorinating microorganisms were detected in the sandstone matrix at both locations, but the detected microbial diversity calculated with PCR-DGGE was significantly higher in samples collected from the core located closer to the source zone, where contaminant concentrations are higher and contaminant compositions more diverse, compared to samples from the plume front location.